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Reference: Almeida, M, Pintacuda, G, Masui, O et al., (2017). PCGF3/5-PRC1 initiates Polycomb recruitment in X chromosome inactivation. Science, 356 (6342), 1081-1084.Citable link to this page:

 

PCGF3/5-PRC1 initiates Polycomb recruitment in X chromosome inactivation

Abstract: Recruitment of the Polycomb repressive complexes PRC1 and PRC2 by Xist RNA is an important paradigm for chromatin regulation by long non-coding RNAs. Here we show that the non-canonical PCGF3/5-PRC1 complex initiates recruitment of both PRC1 and PRC2 in response to Xist RNA expression. PCGF3/5-PRC1 mediated ubiquitylation of histone H2A signals recruitment of other non-canonical PRC1 complexes, and of PRC2, leading to deposition of histone H3 lysine 27 methylation chromosome wide. Pcgf3/5 gene knockout results in female specific embryo lethality, and abrogates Xist mediated gene repression, highlighting a key role for Polycomb in Xist dependent chromosome silencing. Our findings overturn existing models for Polycomb recruitment by Xist RNA, and moreover establish precedence for H2AK119u1 in initiating Polycomb domain formation in a physiological context.

Publication status:PublishedPeer Review status:Peer reviewedVersion:Accepted ManuscriptDate of acceptance:12 May 2017 Funder: Wellcome Trust   Funder: EuropeanResearch Council   Notes:Copyright © 2017 American Association for the Advancement of Science. This is the accepted manuscript version of the article. The final version is available online from American Association for the Advancement of Science at: https://doi.org/10.1126/science.aal2512

Bibliographic Details

Publisher: American Association for the Advancement of Science

Publisher Website: http://www.aaas.org/

Journal: Sciencesee more from them

Publication Website: http://www.sciencemag.org/

Volume: 356

Issue: 6342

Extent: 1081-1084

Issue Date: 2017-06-09

pages:1081-1084Identifiers

Doi: https://doi.org/10.1126/science.aal2512

Uuid: uuid:132032ac-36e3-464f-9fe1-94ee7de7de87

Urn: uri:132032ac-36e3-464f-9fe1-94ee7de7de87

Pubs-id: pubs:697911

Issn: 0036-8075

Eissn: 1095-9203 Item Description

Type: journal-article;

Language: English

Version: Accepted ManuscriptKeywords: Letter

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Autor: Almeida, M - Oxford, MSD, Biochemistry fundingFundação para a Ciência e Tecnologia, Portugal grantNumberSFRH-BD-51706-2011 - -

Fuente: https://ora.ox.ac.uk/objects/uuid:132032ac-36e3-464f-9fe1-94ee7de7de87



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