High efficiency protoplast isolation from in vitro cultures and hairy roots of Maesa lanceolataReportar como inadecuado




High efficiency protoplast isolation from in vitro cultures and hairy roots of Maesa lanceolata - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

(2010)AFRICAN JOURNAL OF BIOTECHNOLOGY.9(42).p.7071-7078 Mark abstract In vitro cultures of the medicinal plant Maesa lanceolata were established to enable the cultivation of plant material for the production of protoplasts. Callus cultures were initiated using leaves collected from shoot cultures and the root tips from hairy root cultures obtained upon Agrobacterium rhizogenes transformation. For the isolation of protoplast, the different explant material of M. lanceolata was exposed to an enzyme mixture consisting of 1.5% cellulase, 0.5% macerozyme R-10 and 0.5 M mannitol.About 6 x 106 protoplasts g-1 fresh weight were obtained from leaf material and 5 x 105 protoplasts g-1 fresh weight from callus. To obtain high amounts of hairy root protoplasts, the cultures were pretreated with the auxin indole-3-butyric acid (IBA) that stimulated the formation of novel root tips. Using the dissected root tips as starting material, 8 x 105 protoplasts g-1 fresh weight were obtained per preparation. The protoplast isolation method will enable further studies on the transformation and fusion of protoplasts from M. lanceolata.

Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-1057768



Autor: Ellen Lambert and Danny Geelen

Fuente: https://biblio.ugent.be/publication/1057768



DESCARGAR PDF




Documentos relacionados