N-Cadherin in neuroblastoma disease : expression and clinical significanceReport as inadecuate




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(2012)PLOS ONE.7(2). Mark abstract One of the first and most important steps in the metastatic cascade is the loss of cell-cell and cell-matrix interactions. N-cadherin, a crucial mediator of homotypic and heterotypic cell-cell interactions, might play a central role in the metastasis of neuroblastoma (NB), a solid tumor of neuroectodermal origin. Using Reverse Transcription Quantitative PCR (RT-qPCR), Western blot, immunocytochemistry and Tissue MicroArrays (TMA) we demonstrate the expression of N-cadherin in neuroblastoma tumors and cell lines. All neuroblastic tumors (n = 356) and cell lines (n = 10) expressed various levels of the adhesion protein. The N-cadherin mRNA expression was significantly lower in tumor samples from patients suffering metastatic disease. Treatment of NB cell lines with the N-cadherin blocking peptide ADH-1 (Exherin, Adherex Technologies Inc.), strongly inhibited tumor cell proliferation in vitro by inducing apoptosis. Our results suggest that N-cadherin signaling may play a role in neuroblastoma disease, marking involvement of metastasis and determining neuroblastoma cell viability.

Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-2127317



Author: Tim Lammens , Katrien Swerts , Lara Derycke , ANNEMIE DE CRAEMER, Sara De Brouwer, Katleen De Preter , Nadine Van Roy , Jo Vandeso

Source: https://biblio.ugent.be/publication/2127317



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