Virulence variation of white spot syndrome virus in Pacific white shrimp Litopenaeus vannamei.Reportar como inadecuado


 Virulence variation of white spot syndrome virus in Pacific white shrimp Litopenaeus vannamei.


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Type of Resource: text

Genre: article

Date Issued: 2009

Publisher: American Fisheries Society.

Place of Publication: Kissimmee, FL

Physical Form: pdf

Extent: 10 p.

Language(s): English

Identifier: FA00007162 (IID)

Note(s): The virulence of seven geographic isolates of white spot syndrome virus (WSSV; genusWhispovirus; China [strain CH1995], Nicaragua [strain N2000], Honduras [strain H2000], Ecuador [strains EL1999and E-LT2002], and Mexico [strains M-M2001 and M-LP2001]) was compared using a series ofchallenge experiments, each lasting 10 d. For each isolate, four quantified dilutions (10 ˉ⁶, 10 ˉ⁷, 10 ˉ⁸, and10 ˉ⁹) of a viral inoculum were prepared from WSSV-infected shrimp tissue. Each viral inoculum was injectedinto 10 specific pathogen-free juvenile Pacific white shrimp Litopenaeus vannamei (0.25–1.50 g); controlsreceived injections of marine crustacean physiological saline (3.2%). The minimum dose of viral inoculumthat killed 50% of injected shrimp (LD50) was calculated for dilution, tissue concentration, and viral DNAamount. The CH1995 and M-M2001 isolates were the least virulent, with LD50 values of 10 ˉ⁶ to 10 ˉ⁷ ofviral inoculum. The isolates could be grouped into three virulence clusters (CH1995 and M-M2001; N2000and E-LT2002; and H2000, E-L1999, and M-LP2001). Virulence clusters were not altered by LD50 valuesbased on viral DNA concentration, although a slight shifting of order in regards to virulence was seen amongthe three most virulent isolates (E-L1999, H2000, and M-LP2001). Overall, results indicate that there is a measurable virulence difference among WSSV isolates, which may correspond to geographical region.Florida Atlantic University. Harbor Branch Oceanographic Institute contribution 1714This manuscript is an author version with the finalpublication available and may be cited as: Laramore, S. E., Scarpa, J., Laramore, C. R., & Lin, J. (2009).Virulence variation of white spot syndrome virus in Pacific white shrimp Litopenaeus vannamei.Journal of Aquatic Animal Health, 21(2), 82-90. doi: 10.1577/H08-012.1

Subject(s): Pacific white shrimpWhiteleg shrimpLitopenaeus vannameiWhite spot syndrome virus 1Virulences

Links: http://dx.doi.org/10.1577/H08-012.1

Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00007162

Owner Institution: FAU



Autor: Laramore, S. E. Scarpa, J. Laramore, C. R. Lin, J.

Fuente: http://fau.digital.flvc.org/islandora/object/fau%3A32731



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