Microbial community analysis of the octocoral Eunice a fusca; combined use of cultivation-dependent and independent techniques. - Download this document for free, or read online. Document in PDF available to download.



Type of Resource: text

Genre: Electronic Thesis or Dissertation

Date Created: 2007

Date Issued: 2007

Publisher: Florida Atlantic University

Physical Form: pdf

Extent: 93 p.

Language(s): English

Abstract/Description: The octocoral Eunicea fusca is the source of important anti-inflammatory compoundssuch as the diterpenes fuscol and the fuscosides. Evidence suggests that thesecompounds are being produce by bacterial sources that live in symbiosis with the coral.As part of an investigation to better understand the role of bacteria associated with E.fusca , the characterization of the bacterial community using two different techniques(culture dependent technique and culture independent technique) and sea water sampleswas done. Sea water samples were used as a control to determine how closely associatedbacteria are to this octocoral. The polymerase chain reaction (PCR) with universalbacterial primers FC27 and RC 1492 specific to prokaryotic 16S rDNA gene sequenceswas used to characterize the total bacterial population when using both the culturable andthe non-culturable approaches. The results indicate a diverse group of bacteria associated to E. fusca composed of 10 different groups. The pro teo bacteria group was the mostpredominant group when both techniques were used. The a-proteobacteria representedthe highest percentage of bacteria associated to E. fusca.

Identifier: FA00000748 (IID)

Note(s): Includes bibliography.Thesis (M.S.)--Florida Atlantic University, 2007.

Held by: Florida Atlantic University Digital Library

Sublocation: Boca Raton, Fla.

Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00000748

Restrictions on Access: All rights reserved by the source institution

Owner Institution: FAU



Author: Duque-Alarcon, Angela Patricia, author Kerr, Russell Dr., Thesis advisor Charles E. Schmidt College of Science, Degree grantor

Source: http://fau.digital.flvc.org/islandora/object/fau%3A34306

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