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Juliana Silveira do Valle ; Ezilda Jacomassi ; Giani Andrea Linde ; Nelson Barros Colauto ;Semina: Ciências Agrárias 2013, 34 2

Autor: Sandra Sayuri Nakamura

Fuente: http://www.redalyc.org/articulo.oa?id=445744120014


Introducción



Semina: Ciências Agrárias ISSN: 1676-546X semina.agrarias@uel.br Universidade Estadual de Londrina Brasil Sayuri Nakamura, Sandra; Silveira do Valle, Juliana; Jacomassi, Ezilda; Linde, Giani Andrea; Barros Colauto, Nelson Molecular authentication of Maytenus sp by PCR-RFLP Semina: Ciências Agrárias, vol.
34, núm.
2, marzo-abril, 2013, pp.
627-633 Universidade Estadual de Londrina Londrina, Brasil Available in: http:--www.redalyc.org-articulo.oa?id=445744120014 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative DOI: 10.5433-1679-0359.2013v34n2p627 Molecular authentication of Maytenus sp by PCR-RFLP Autenticação molecular de Maytenus sp por PCR-RFLP Sandra Sayuri Nakamura1; Juliana Silveira do Valle2; Ezilda Jacomassi2; Giani Andrea Linde3; Nelson Barros Colauto3* Abstract Maytenus aquifolia and Maytenus ilicifolia are native plants from South America and popularly known as ‘Espinheira-santa’.
Both are used as tea due to their ef ciency in the treatment of ulcer, gastritis and indigestion.
However, adulteration of processed Maytenus genus tea with Sorocea genus may happen due to their botanical similarity, compromising the quality of the products and opening a derogatory business opportunity that may lead to the discrediting of medicinal plant products.
This study aimed to distinguish Maytenus sp and Sorocea bonplandii by PCR-RFLP of a chloroplast DNA (cpDNA) intergenic region.
Three commercial products of processed tea leaves of Maytenus sp, and in natura leaves of Maytenus sp and S.
bonplandii were analyzed.
PCR detected unique fragments for all samples in natura.
The trnH-psbA region amplicon of both M.
ilicifolia and M.
aquifolia was 660 bp, and for S.
bonplandii was 565 bp.
These PCR products can be used as markers t...





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