DNA heats up : Energetics of genome ejection from phage revealed by isothermal titration calorimetry - Physics > Biological PhysicsReportar como inadecuado




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Abstract: Most bacteriophages are known to inject their double-stranded DNA intobacteria upon receptor binding in an essentially spontaneous way. This downhillthermodynamic process from the intact virion toward the empty viral capsid plusreleased DNA is made possible by the energy stored during active packaging ofthe genome into the capsid. Only indirect measurements of this energy have beenavailable until now using either single-molecule or osmotic suppressiontechniques. In this paper, we describe for the first time the use of isothermaltitration calorimetry to directly measure the heat released or equivalentlythe enthalpy during DNA ejection from phage lambda, triggered in solution by asolubilized receptor. Quantitative analyses of the results lead to theidentification of thermodynamic determinants associated with DNA ejection. Thevalues obtained were found to be consistent with those previously predicted byanalytical models and numerical simulations. Moreover, the results confirm therole of DNA hydration in the energetics of genome confinement in viral capsids.



Autor: Meerim Jeembaeva L.U., CMU, B. Jönsson L.U., Martin Castelnovo Phys-ENS, Alex Evilevitch L.U., CMU

Fuente: https://arxiv.org/







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