Transcriptome analysis uncovers Arabidopsis F-BOX STRESS INDUCED 1 as a regulator of jasmonic acid and abscisic acid stress gene expressionReport as inadecuate




Transcriptome analysis uncovers Arabidopsis F-BOX STRESS INDUCED 1 as a regulator of jasmonic acid and abscisic acid stress gene expression - Download this document for free, or read online. Document in PDF available to download.

BMC Genomics

, 18:533

Plant genomics

Abstract

BackgroundThe ubiquitin 26S proteasome system UPS selectively degrades cellular proteins, which results in physiological changes to eukaryotic cells. F-box proteins are substrate adaptors within the UPS and are responsible for the diversity of potential protein targets. Plant genomes are enriched in F-box genes, but the vast majority of these have unknown roles. This work investigated the Arabidopsis F-box gene F-BOX STRESS INDUCED 1 FBS1 for its effects on gene expression in order elucidate its previously unknown biological function.

ResultsUsing publically available Affymetrix ATH1 microarray data, we show that FBS1 is significantly co-expressed in abiotic stresses with other well-characterized stress response genes, including important stress-related transcriptional regulators. This gene suite is most highly expressed in roots under cold and salt stresses. Transcriptome analysis of fbs1–1 knock-out plants grown at a chilling temperature shows that hundreds of genes require FBS1 for appropriate expression, and that these genes are enriched in those having roles in both abiotic and biotic stress responses. Based on both this genome-wide expression data set and quantitative real-time PCR qPCR analysis, it is apparent that FBS1 is required for elevated expression of many jasmonic acid JA genes that have established roles in combatting environmental stresses, and that it also controls a subset of JA biosynthesis genes. FBS1 also significantly impacts abscisic acid ABA regulated genes, but this interaction is more complex, as FBS1 has both positive and negative effects on ABA-inducible and ABA-repressible gene modules. One noteworthy effect of FBS1 on ABA-related stress processes, however, is the restraint it imposes on the expression of multiple class I LIPID TRANSFER PROTEIN LTP gene family members that have demonstrated protective effects in water deficit-related stresses.

ConclusionFBS1 impacts plant stress responses by regulating hundreds of genes that respond to the plant stress hormones JA and ABA. The positive effect that FBS1 has on JA processes and the negative effect it has on at least some ABA processes indicates that it in part regulates cellular responses balanced between these two important stress hormones. More broadly then, FBS1 may aid plant cells in switching between certain biotic JA and abiotic ABA stress responses. Finally, because FBS1 regulates a subset of JA biosynthesis and response genes, we conclude that it might have a role in tuning hormone responses to particular circumstances at the transcriptional level.

KeywordsJasmonic acid Abscisic acid F-box Plant stress AbbreviationsABAAbscisic acid

AOCALLENE OXIDE CYCLASE

AOSALLENE OXIDE SYNTHASE

ASK1Arabidopsis Skp1

BARBio-analytic resource

CBFC-repeat binding factor

ERFETHYLENE RESPONSE FACTOR

FBS1F-BOX STRESS INDUCED 1

GAGibberellic acid

GOGene ontology

IAAIndole-3-acetic acid

JAJasmonic acid

JA-IleJasmonate-isoleucine

JAZJASMONATE ZIM-DOMAIN

LC-MSLiquid chromatography-mass spectrometry

LOXLIPOXYGENASE

LTPLIPID TRANSFER PROTEIN

MeJAMethyl jasmonate

NRQNormalized relative quantity

OPR12-OXOPHYTODIENOIC ACID REDUCTASE

qPCRQuantitative real-time polymerase chain reaction

RMARobust multi-array

RNA-seqRNA-sequencing

ROSReactive oxygen species

RT-PCRReverse transcription polymerase chain reaction

SASalicylic acid

SCFSkp1-Cullin-F-box

UPSUbiquitin 26S proteasome system

WTWild type

Electronic supplementary materialThe online version of this article doi:10.1186-s12864-017-3864-6 contains supplementary material, which is available to authorized users.

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Author: Lauren E. Gonzalez - Kristen Keller - Karen X. Chan - Megan M. Gessel - Bryan C. Thines

Source: https://link.springer.com/







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