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Virology Journal

, 14:144

Positive-strand RNA viruses


BackgroundEchovirus 30 E30 causes acute aseptic meningitis. Viral replication requires energy and macromolecular precursors derived from the metabolic network of the host cell. The effect of viral infection within a host cell metabolic activity remains unclear.

MethodsTo gain an insight into cell-virus interaction during E30 infection we used a human rhabdomyosarcoma cell line. In a new approach to metabolomics, NMR was used to measure the level of various cellular metabolites at different times of infection and morphological examination of the cells. Statistical analysis was done by using Confidence interval CI 95% and One-way ANOVA test.

ResultsTheH NMR metabolite spectrum signals were observed between mock infected and virus infected cells. Both mock infected and virus infected cells utilized glucose through metabolic pathways and released metabolic end products. Upon infection, the concentration of Alanine, Lactate, Acetate, Glutamate, Tyrosine, Histidine, Phenylalanine, Creatine, Choline and Formate, increased. Interestingly, all of these augmented metabolites were decreased during later stage of infection. The cells showed wide-ranging lipid signals at the end of infection, which correlates with the morphological changes as apoptosis programmed cell death of cells was observed. A significant association was found between time interval 12 h, 24 h, and 48 h and metabolites likewise Alanin, Lactate, Acetate, Glutamate, Tyrosine, Histidine, Phenylalanine, Creatine, Choline and Formate respectively released by cell during infection, which is highly significant p < 0.01.

ConclusionProgressive breakdown and utilization of all cellular components were observed as the infection increased. This study is useful for monitoring the cellular metabolic changes during viral infection.

KeywordsEchovirus 30 NMR Cellular metabolomics Aseptic meningitis Tissue culture AbbreviationsCO2Carbon Dioxide

D2ODeuterium Oxide

E30Echovirus 30


FBSFetal Bovine Serum

HClHydrochloric acid

HEPESN-2-hydroxyethylpiperazine-N0-2-ethanesulfonic acid

KClPotassium Chloride

MEMMinimum Essential Medium

MgCl2Magnesium Chloride

NMRNuclear Magnetic Resonance

PBSPhosphate Buffered Saline

PFUPlaque Forming Unit


TSPTrimethylSilylPropionic acid

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Autor: Sarika Tiwari - Tapan N. Dhole


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