Effects of IFN-β1a and IFN-β1b treatment on the expression of cytokines, inducible NOS NOS type II, and myelin proteins in animal model of multiple sclerosisReportar como inadecuado




Effects of IFN-β1a and IFN-β1b treatment on the expression of cytokines, inducible NOS NOS type II, and myelin proteins in animal model of multiple sclerosis - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Archivum Immunologiae et Therapiae Experimentalis

, Volume 65, Issue 4, pp 325–338

First Online: 15 March 2017Received: 03 July 2016Accepted: 09 February 2017

Abstract

The aim of this study was to investigate the effects of interferon IFN-β1a and IFN-β1b treatment on inflammatory factors and myelin protein levels in the brain cortex of the Lewis rat experimental autoimmune encephalomyelitis EAE, animal model of multiple sclerosis. To induce EAE, rat were immunized with inoculums containing spinal cord guinea pig homogenized in phosphate-buffered saline and emulsified in Freund’s complete adjuvant containing 110 µg of the appropriate antigen in 100 µl of an emulsion and additionally 4-mg-ml Mycobacterium tuberculosis H37Ra. The rats were treated three times per week with subcutaneous applications of 300,000 units IFN-β1a or IFN-β1b. The treatments were started 8 days prior to immunization and continued until day 14 after immunization. The rats were killed on the 14th day of the experiment. EAE induced dramatic increase in interleukin IL-1β, IL-6, and tumor necrosis factor TNF-concentrations and inducible nitric oxide synthase iNOS expression in the brain, which closely corresponded to the course of neurological symptoms and the loss of weight. Both IFN-β1b and IFN-β1a treatments inhibited the pro-inflammatory cytokines IL-6, IL-1β, TNF-α and IFN-γ, decreased the activation of astrocytes, increased the myelin protein level in the brain cortex, and improved the neurological status of EAE rats by different mechanisms; IFN-β1a reduced iNOS expression, at least in part, by the enhancement of IL-10, while IFN-β1b diminished IL-10 concentration and did not decrease EAE-induced iNOS expression.

KeywordsInterferon beta Cytokines Inducible nitric oxide synthase EAE  Download fulltext PDF



Autor: Natalia Lubina-Dąbrowska - Adam Stepień - Grzegorz Sulkowski - Beata Dąbrowska-Bouta - Józef Langfort - Małgorzata Chal

Fuente: https://link.springer.com/







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