Genetic structure and relationships within and between cultivated and wild korarima Aframomum corrorima Braun P.C.M. Jansen in Ethiopia as revealed by simple sequence repeat SSR markersReportar como inadecuado




Genetic structure and relationships within and between cultivated and wild korarima Aframomum corrorima Braun P.C.M. Jansen in Ethiopia as revealed by simple sequence repeat SSR markers - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Genetics

, 18:72

Plant population genetics

Abstract

BackgroundKorarima Aframomum corrorima Braun P.C.M. Jansen is a spice crop native to Ethiopia. Understanding the extent and partitioning of diversity within and among crop landraces and their wild relatives is among the first steps in conserving and measuring their genetic potential. The present study is aimed at characterizing the population genetic structure and relationships between cultivated and wild korarima in the southwestern part of Ethiopia.

ResultsWe analyzed a total of 195 individuals representing seven wild and fourteen cultivated populations. Eleven polymorphic simple sequence repeat SSR markers were used. We observed a total of 53 alleles across the eleven loci and individuals. In total, 32 alleles were detected in the cultivated populations, whereas 49 alleles were detected in the wild populations. We found higher genetic diversity in wild populations than in the cultivated counterpart. This result implies the potential of wild korarima as a possible source for novel alleles contributing to the improvement of cultivated korarima. Analysis of molecular variance AMOVA showed significant but low differentiation between cultivated and wild korarima populations. Similarly, neighbour-joining and STRUCTURE analyses did not group cultivated and wild populations into two distinct clusters. The lack of clear differentiation between cultivated and wild populations could be explained by historical and contemporary gene flow between the two gene pools.

ConclusionThe 11 SSR loci developed in this study could be employed to examine genetic diversity and population structure of korarima in other countries as well as other Aframomum species. From the five administrative zones considered in this study, the Bench-Magi and Sheka zone showed populations with high genetic diversity, and these populations could be used as a potential starting point for in-situ and ex-situ germplasm conservation and korarima improvement through breeding programs after proper agronomic evaluation.

KeywordsAframomum corrorima Korarima Genetic diversity Simple sequence repeats Genetic structure Genetic differentiation Gene flow AbbreviationsAMOVAAnalysis of molecular variance

CTABCetyl trimethyl ammonium bromide

ESTExpressed sequence tags

IBCInstitute of Biodiversity Conservation

NCBINational Center for Biotechnology information

SNNPSouthern Nations, Nationalities, and Peoples’ Region

SSRSimple sequence repeats

Electronic supplementary materialThe online version of this article doi:10.1186-s12863-017-0540-4 contains supplementary material, which is available to authorized users.

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Autor: Dagmawit Chombe - Endashaw Bekele - Tomas Bryngelsson - Abel Teshome - Mulatu Geleta

Fuente: https://link.springer.com/



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