Prospecting Agro-waste Cocktail: Supplementation for Cellulase Production by a Newly Isolated Thermophilic B. licheniformis 2D55Reportar como inadecuado




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Applied Biochemistry and Biotechnology

, Volume 182, Issue 4, pp 1318–1340

First Online: 07 February 2017Received: 19 October 2016Accepted: 09 January 2017

Abstract

Bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. The cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for biofuel production. Among three potential bacteria examined, Bacillus licheniformis 2D55 accession no. KT799651 was found to produce the highest cellulolytic activity CMCase 0.33 U-mL and FPase 0.09 U-mL at 18–24 h fermentation when grown on microcrystalline cellulose MCC as a carbon source in shake flask at 50 °C. Cellulase production process was further conducted on the untreated and NaOH pretreated rice straw RS, rice husk RH, sugarcane bagasse BAG and empty fruit bunch EFB. Untreated BAG produced the highest FPase 0.160 U-mL, while the highest CMCase 0.150 U-mL was supported on the pretreated RH. The mixture of untreated BAG and pretreated RH as agro-waste cocktail has remarkably improved CMCase 3.7- and 1.4-fold and FPase 2.5- and 11.5-fold compared to the untreated BAG and pretreated RH, respectively. The mechanism of cellulase production explored through SEM analysis and the location of cellulase enzymes of the isolate was also presented. Agro-waste cocktail supplementation provides an alternative method for an efficient production of cellulase.

KeywordsComposting Thermophilic bacteria Cellulase production Agro-waste cocktail Enzyme location NaOH pretreatment Scanning electron micrograph SEM  Download fulltext PDF



Autor: Muinat Olanike Kazeem - Umi Kalsom Md Shah - Azhari Samsu Baharuddin - Nor’ Aini AbdulRahman

Fuente: https://link.springer.com/



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