Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transferReportar como inadecuado

Genome-wide microRNA expression profiling in placentae from frozen-thawed blastocyst transfer - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Clinical Epigenetics

, 9:79

All other subjects


BackgroundFrozen-thawed embryo transfer FET is increasingly available for the improvement of the success rate of assisted reproductive technologies other than fresh embryo transfer ET. There have been numerous findings that FET provides better obstetric and perinatal outcomes. However, the birth weight of infants conceived using FET is heavier than that of those conceived via ET. In addition, some reports have suggested that FET is associated with perinatal diseases such as placenta accreta and pregnancy-induced hypertension PIH.

ResultsIn this study, we compared the microRNA miRNA expression profiles in term placentae derived from FET, ET, and spontaneous pregnancy SP. We identified four miRNAs, miR-130a-3p, miR-149-5p, miR-423-5p, and miR-487b-3p, that were significantly downregulated in FET placentae compared with those from SP and ET. We found that DNA methylation of MEG3-DMR, not but IG-DMR, was associated with miRNA expression of the DLK1-DIO3 imprinted domain in the human placenta. In functional analyses, GO terms and signaling pathways related to positive regulation of gene expression, growth, development, cell migration, and type II diabetes mellitus T2DM were enriched.

ConclusionsThis study supports the hypothesis that the process of FET may increase exposure of epigenome to external influences.

KeywordsAssisted reproductive technologies ART Frozen-thawed embryo transfer FET Placenta MicroRNAs miRNAs Microarray Real-time PCR AbbreviationsARTAssisted reproductive technology

BWSBeckwith–Wiedemann syndrome

C14MCChromosome 14 microRNA cluster

C19MCChromosome 19 microRNA cluster

COBRACombined bisulfite restriction analysis

DAVIDDatabase for Annotation, Visualization and Integrated Discovery

DMDiabetes mellitus

ETEmbryo transfer

FETFrozen-thawed embryo transfer

FSHFollicular stimulating hormone

GnRHGonadotropin-releasing hormone

GOGene ontology

hCGHuman chorionic gonadotropin

hMGHuman menopausal gonadotropin

HRCHormone replacement cycle

ICSIIntracytoplasmic sperm injection

IVFIn vitro fertilization

KEGGKyoto Encyclopedia of Genes and Genomes

LBWLow birthweight

LGALarge for gestational age

LN2Liquid nitrogen

MAPKMitogen-activated protein kinase

PIHPregnancy-induced hypertension

rFSHRecombinant follicular stimulating hormone

RT-PCRReverse transcription polymerase chain reaction

SDStandard deviation

SGASmall for gestational age

SPSpontaneous pregnancy

SRSSilver-Russell syndrome

T2DMType 2 diabetes mellitus

VEGFVascular endothelial growth factor

Electronic supplementary materialThe online version of this article doi:10.1186-s13148-017-0379-6 contains supplementary material, which is available to authorized users.

Download fulltext PDF

Autor: Hitoshi Hiura - Hiromitsu Hattori - Norio Kobayashi - Hiroaki Okae - Hatsune Chiba - Naoko Miyauchi - Akane Kitamura - Hiro


Documentos relacionados