Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component GC gene in clinical mastitis and milk productionReportar como inadecuado




Fine mapping of a QTL on bovine chromosome 6 using imputed full sequence data suggests a key role for the group-specific component GC gene in clinical mastitis and milk production - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Genetics Selection Evolution

, 48:79

First Online: 19 October 2016Received: 22 December 2015Accepted: 12 October 2016

Abstract

BackgroundClinical mastitis is an inflammation of the mammary gland and causes significant costs to dairy production. It is unfavourably genetically correlated to milk production, and, thus, knowledge of the mechanisms that underlie these traits would be valuable to improve both of them simultaneously through breeding. A quantitative trait locus QTL that affects both clinical mastitis and milk production has recently been fine-mapped to around 89 Mb on bovine chromosome 6 BTA6, but identification of the gene that underlies this QTL was not possible due to the strong linkage disequilibrium between single nucleotide polymorphisms SNPs within this region. Our aim was to identify the gene and, if possible, the causal polymorphisms responsible for this QTL through association analysis of high-density SNPs and imputed full sequence data in combination with analyses of transcript and protein levels of the identified candidate gene.

ResultsAssociations between SNPs and the studied traits were strongest for SNPs that were located within and immediately upstream of the group-specific component GC gene. This gene encodes the vitamin D-binding protein DBP and has multiple roles in immune defense and milk production. A 12-kb duplication that was identified downstream of this gene covered its last exon and segregated with the QTL allele that is associated with increased mastitis susceptibility and milk production. However, analyses of GC mRNA levels on the available samples revealed no differences in expression between animals having or lacking this duplication. Moreover, we detected no differences in the concentrations of DBP and its ligand vitamin D between the animals with different GC genotypes that were available for this study.

ConclusionsOur results suggest GC as the gene that underlies the QTL for clinical mastitis and milk production. However, since only healthy animals were sampled for transcription and expression analyses, we could not draw any final conclusion on the absence of quantitative differences between animals with different genotypes. Future studies should investigate GC RNA expression and protein levels in cows with different genotypes during an infection.

Tim Martin Knutsen and Anna M. Lewandowska-Sabat contributed equally to this work

Electronic supplementary materialThe online version of this article doi:10.1186-s12711-016-0257-2 contains supplementary material, which is available to authorized users.

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Autor: Hanne Gro Olsen - Tim Martin Knutsen - Anna M. Lewandowska-Sabat - Harald Grove - Torfinn Nome - Morten Svendsen - Marian

Fuente: https://link.springer.com/







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