Permeability and distribution of nerve growth factor in the brain of neonatal rats by periphery venous injection in hypoxic-ischemic stateReportar como inadecuado




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SpringerPlus

, 5:1893

Medicine

Abstract

ObjectiveTo investigate the permeability of β-NGF through blood–brain-barrier BBB in neonatal and adult rats, and the spatial distribution of β-NGF in different brain regions in hypoxic-ischemic HI and normal neonatal rats.

MethodsTo investigate the overall permeability of β-NGF through BBB, β-NGF labeled with I was injected into adult rats, neonatal rats and HI neonatal rats via tail vein. The radioactivity of brain tissue and blood was examined and analyzed 30 min after injection. Also, brain regions including the basal forebrain, frontal cortex, hippocampus, hypothalamus, cerebellum, bulbus olfactorius and hypophysis, of all the rats were dissected and radioactivity was examined to investigate the spatial specificity of NGF permeation through BBB.

ResultsStatistically significant results were observed in I-β-NGF contents in brain tissues of adult rats group, neonatal rats group and HI neonatal rats group P < 0.05. Compared to the HI neonatal rats’ brain with the highest I-β-NGF contents, normal neonatal rats ranks the second while the adult rats were the lowest. While for the spatial specificity examination part, I-β-NGF in both HI group and control group were widely distributed in basal forebrain, frontal cortex, hippocampus, cerebellum and bulbus olfactorius. But the radioactivity in frontal cortex, hippocampus and cerebellum of HI groups are statistically higher than control groups P < 0.05.

Conclusionβ-NGF can more easily penetrate the BBB of newborn rats than adult rats via peripheral venous administration and this effect can be enhanced by HI insult. Also, this HI-induced permeation of β-NGF through BBB is more obvious in frontal cortex, hippocampus and cerebellum.

KeywordsNerve growth factor Hypoxic Ischemic Blood–brain barrier AbbreviationsBBBblood-brain-barrier

HIhypoxia-ischemia

BFbasal forebrain

CFfrontal cortex

CBcerebellum

HFhypocampus

HYhypothalamus

OBbulbus olfactorius

PIhypophysis

Wenli Zhou and Jiantao Zhang contributed equally to this work

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Autor: Wenli Zhou - Jiantao Zhang - Guangming Wang - Limian Ling - Chaoying Yan

Fuente: https://link.springer.com/







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