Development and validation of an assay for measurement of leptin in pig salivaReportar como inadecuado




Development and validation of an assay for measurement of leptin in pig saliva - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Veterinary Research

, 12:242

Clinical pathology, physiology and immunology

Abstract

BackgroundLeptin has been measured in human in saliva samples. However, the low leptin concentration found in this biological fluid makes necessary the use of high sensitive methods. To the authors’ knowledge, leptin has not been measured in porcine saliva. This study aimed to develop and validate a time-resolved immunofluorometric assay TR-IFMA for salivary leptin measurements in pigs, using a species-specific antibody, and to evaluate how salivary leptin changes with body weight, food ingestion, and in experimental models of stress and inflammation. Polyclonal antibodies were produced in rabbits immunized with recombinant porcine leptin and used to develop a sandwich TR-IFMA.

ResultsThe method had intra-assay and inter-assay coefficients of variation lower than 10 and 16 %, respectively. The assay was accurate and the low limit of detection allowed detection of leptin in all analyzed samples. Salivary leptin concentration was positively correlated to body weight r = 0.58, P = 0.01 and increased after food ingestion P < 0.001 and after 24 h of applying a model of experimental inflammation by turpentine injection P < 0.05. However, it did not significantly change after a model of acute stress consisting of a nose snare restraining.

ConclusionThese results indicate that the developed assay can measure leptin in porcine saliva in a reliable way and that leptin in saliva is influenced by body weight, food ingestion and inflammation.

KeywordsInflammation Leptin Porcine saliva Stress Validation AbbreviationsCgAChromogranin A

CRPC-reactive protein

CVCoefficient of variation

EuEuropium

IgGImmunoglobulin G

RIARadioimmunoassay

SDS-PAGESodium dodecyl sulphate polyacrilamide gel electrophoresis

TR-IFMATime resolved immunofluorometric assay

Download fulltext PDF



Autor: Elizabeth M. S. Schmidt - Damián Escribano - Silvia Martinez-Subiela - Silvia Martinez-Miró - Fuensanta Hernández - Asta

Fuente: https://link.springer.com/



DESCARGAR PDF




Documentos relacionados