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BMC Ophthalmology

, 16:191

Cornea and external eye diseases


BackgroundThe repertoire of free-living protozoa in contact lens solutions is poorly known despite the fact that such protozoa may act as direct pathogens and may harbor intra-cellular pathogens.

MethodsBetween 2009 and 2014, the contact lens solutions collected from patients presenting at our Ophthalmology Department for clinically suspected keratitis, were cultured on non-nutrient agar examined by microscope for the presence of free-living protozoa. All protozoa were identified by 18S rRNA gene sequencing.

ResultsA total of 20 of 233 8.6 % contact lens solution specimens collected from 16 patients were cultured. Acanthamoeba amoeba in 16 solutions 80 % collected from 12 patients and Colpoda steini, Cercozoa sp., Protostelium sp. and a eukaryotic more closely related to Vermamoeba sp., were each isolated in one solution. Cercozoa sp., Colpoda sp., Protostelium sp. and Vermamoeba sp. are reported for the first time as contaminating contact lens solutions.

ConclusionThe repertoire of protozoa in contact lens solutions is larger than previously known.

KeywordsAcanthamoeba Keratitis Contact lens solution Protozoa  Download fulltext PDF

Autor: Ibtissem Bouchoucha - Aurore Aziz - Louis Hoffart - Michel Drancourt


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