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BMC Veterinary Research

, 12:243

Gastroenterology and nutrition

Abstract

BackgroundChitosan oligosaccharide COS is widely consumed as a functional food due to its multiple health effects, but few studies about COS supplement on placental antioxidant and nutrition transport capacity were reported. Taken pregnant sow as a model, we aimed to investigate the effects of dietary COS supplementation during late gestation on placental amino acids transport and antioxidant defense capacity of sows. From day d 85 of gestation to parturition, sixteen pregnant sows were divided into a control group basal diet without COS supplementation and a COS group 30 mg COS-kg basal diet. Plasma sample of sow was collected on d 110 of gestation, and placenta tissue was obtained during parturition. Then plasma antioxidant enzyme’s activities, the relative level of oxidant stress related genes, amino acids transport related genes and mTOR pathway molecules in placenta were determined.

ResultsResults showed that maternal dietary supplementation with COS increased P < 0.05 plasma total SOD, caused a downtrend in plasma MDA 0.05 < P < 0.10 on d 110 of gestation. Interestingly, the mRNA expression of some antioxidant genes in the placenta were increased P < 0.05 and pro-inflammatory cytokines were reduced P < 0.05 by COS supplement, whereas no significant difference was observed in the activities of placental total SOD and CAT between two groups. Additionally, further study demonstrated that COS feeding stimulated mTOR signaling pathway, increased amino acids transporters expression in placenta.

ConclusionsThese observations suggested that COS supplement in sow’s diet during late gestation enhanced antioxidant defense capacity of sows, promoted placental amino acids transport, which may contribute to the health of sows and development of fetus during gestation.

KeywordsChitosan oligosaccharides Antioxidant defense Amino acids transport Sow Abbreviation4E-BP1The initiation factor 4E-binding protein 1

ASCT2Alanine-serine-cysteine-threonine transporter

CATCatalase

COSChitosan oligosaccharide

EAATGlutamate transporter

GSH-PxGlutathione peroxidase

LAT1L-type amino-acid transporter 1

MDAMalondialdehyde

mTORMammalian target of rapamycin

p70S6KPhosphorylates the ribosomal p70S6 kinase

PATProton-assisted amino acid transporter

PEPTPeptide transporter

Per1Negative-regulated element period 1, CLOCK, circadian locomotor output cycles kaput

ROSReactive oxygen species

SNATSodium-coupled neutral amino acid transporter

T-SODTotal superoxide dismutase

Ucp2Uncoupling protein

VEGF-AVascular endothelial growth factor A

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Autor: Chunyan Xie - Xin Wu - Cimin Long - Qinhua Wang - Zhiyong Fan - Siming Li - Yulong Yin

Fuente: https://link.springer.com/







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