Differences in host breed and diet influence colonization by Campylobacter jejuni and induction of local immune responses in chickenReportar como inadecuado




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Gut Pathogens

, 8:56

First Online: 10 November 2016Received: 13 September 2016Accepted: 19 October 2016

Abstract

BackgroundChickens are regarded as the main reservoir for human campylobacteriosis. Little is known about the interaction between Campylobacter jejuni C. jejuni and chickens. This interaction may be influenced by the stage of maturation of the immune system, developing gut microbiota composition and other factors including breed and diet. Our aim was to investigate the impact of breed, and diet on C. jejuni colonization and host immune responses in chickens. Birds were inoculated with 10 colony forming units CFU of C. jejuni or diluent at one Exp. 1 or 22 Exp. 2 days post hatch. We compared local immune cell subpopulations, cytokine expression levels, and gut microbiota composition between broiler-type BT and layer-type LT birds fed with either commercial broiler feed bf or layer feed lf.

ResultsLower colonization rates were observed in the older age group independent of breed and diet. Independent of breed, birds fed with bf showed higher CFU of C. jejuni compared to lf-fed groups. Campylobacter jejuni-inoculation had a significant effect on lymphocyte numbers and cytokine expression levels in BT birds independent of feeding strategy p < 0.05. These effects were not detected in LT birds, only LT birds fed with bf showed a significant increase in IL-8-expression at 7 days post C. jejuni inoculation compared to LT-control birds p < 0.05. Diet influenced gut microbiota composition in a comparable manner between BT and LT birds, but changes in microbiota composition associated with C. jejuni inoculation varied between breeds.

ConclusionsDiet and breed influenced C. jejuni colonization, immune responses and microbiota composition to a different extent comparing between LT and BT birds. The mechanisms behind these differences have to be elucidated further. Our results suggest that selection for more resistant breeds in combination with adapted feeding strategies may help to reduce Campylobacter colonization levels in commercial poultry in the future.

KeywordsCampylobacter jejuni Breed Diet Immune response Gut microbiota AbbreviationsC. jejuniCampylobacter jejuni

BTbroiler-type

LTlayer-type

bfbroiler feed

lflayer feed

CFUcolony forming units

dphdays post hatch

ILinterleukin

CCDAcharcoal cefoperazone dexoxycholate agar

PBSphosphate buffered saline

IELintraepithelial lymphocyte

OTUoperational taxonomic units

PCoAprincipal coordinate analysis

dpidays post inoculation

LPLlamina propria lymphocyte

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Autor: Zifeng Han - Thomas Willer - Colin Pielsticker - Lenka Gerzova - Ivan Rychlik - Silke Rautenschlein

Fuente: https://link.springer.com/







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