Detection of the first G6P14 human rotavirus strain in an infant with diarrhoea in GhanaReportar como inadecuado




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Virology Journal

, 13:183

First Online: 10 November 2016Received: 05 August 2016Accepted: 04 November 2016

Abstract

BackgroundRotaviruses with G6P14 specificity are mostly isolated in cattle and have been established as a rare cause of gastroenteritis in humans. This study reports the first detection of G6P14 rotavirus strain in Ghana from the stool of an infant during a hospital-based rotavirus surveillance study in 2010.

MethodsViral RNA was extracted and rotavirus VP7 and VP4 genes amplified by one step RT-PCR using gene-specific primers. The DNA was purified, sequenced and genotypes determined using BLAST and RotaC v2.0. Phylogenetic tree was constructed using maximum likelihood method in MEGA v6.06 software and statistically supported by bootstrapping with 1000 replicates. Phylogenetic distances were calculated using the Kimura-2 parameter model.

ResultsThe study strain, GHA-M0084-2010 was characterised as G6P14. The VP7 gene of the Ghanaian strain clustered in G6 lineage-III together with artiodactyl and human rotavirus HRV strains. It exhibited the highest nucleotide 88.1 % and amino acid 86.9 % sequence identity with Belgian HRV strain, B10925. The VP8* fragment of the VP4 gene was closely related to HRV strains detected in France, Italy, Spain and Belgium. It exhibited the strongest nucleotide sequence identity 87.9 % with HRV strains, PA169 and PR-1300 Italy and the strongest amino acid sequence identity 89.3 % with HRV strain R2775-FRA-07 France.

ConclusionThe study reports the first detection of G6P14 HRV strain in an infant in Ghana. The detection of G6P14, an unusual strain pre-vaccine introduction in Ghana, suggests a potential compromise of vaccine effectiveness and indicates the necessity for continuous surveillance in the post vaccine era.

KeywordsRotavirus Gastroenteritis Genotyping Ghana AbbreviationsaaAmino acid

BLASTBasic local alignment search tool

bpBase pair

EIAEnzyme immunoassay

ELISAEnzyme linked immuno-sorbent assay

GGlycoprotein

HRVAHuman rotavirus group A

MEGAMolecular evolutionary genetics analysis

ntNucleotide

PProtease-sensitive protein

PAGEPolyacrylamide gel electrophoresis

RNARibonucleic acid

RRLRegional reference laboratory

RT-PCRReverse transcription-polymerase chain reaction

RVRotavirus

RVARotavirus group A

VP4Viral protein 4

VP7Viral protein 7

VP8*Viral protein 8*

Electronic supplementary materialThe online version of this article doi:10.1186-s12985-016-0643-y contains supplementary material, which is available to authorized users.

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Autor: Susan Damanka - Belinda Lartey - Chantal Agbemabiese - Francis E. Dennis - Theophilus Adiku - Kofi Nyarko - Michael Ofori

Fuente: https://link.springer.com/







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