Effect of bradykinin on TGF-β1-induced retinal pigment epithelial cell proliferation and extracellular matrix secretionReportar como inadecuado




Effect of bradykinin on TGF-β1-induced retinal pigment epithelial cell proliferation and extracellular matrix secretion - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Ophthalmology

, 16:199

Retina

Abstract

BackgroundTo evaluate the effect of bradykinin BK on TGF-β1-induced retinal pigment epithelial RPE cell proliferation and extracellular matrix secretion and to elucidate the relationship between BK and the Erk-Akt signaling pathway.

MethodsThe effects of BK on TGF-β1-induced RPE cell proliferation were examined via CCK-8 assay. Cell culture supernatant collagen I concentrations were measured via ELISA. Fibronectin Fn, matrix metalloproteinase-2 MMP-2 and MMP-9 mRNA and protein expression levels were measured via q-PCR and Western blotting, respectively. Changes in Akt-Erk phosphorylation induced by BK and HOE-140 were evaluated via Western blotting.

ResultsTGF-β1 stimulated ARPE-19 cell proliferation, which was inhibited by BK, whose effects were inhibited by HOE-140. BK inhibited TGF-β1-induced collagen I, Fn and MMP-2 secretion in RPE cells, and these effects were inhibited by HOE-140. BK also inhibited TGF-β1-induced Akt phosphorylation in RPE cells, and these effects were blocked by HOE-140. BK had no significant effect on Erk-mediated signaling.

ConclusionsThe findings from this study indicate that BK could be novel therapeutic targets for the treatment of PVR.

KeywordsProliferative vitreoretinopathy Retinal pigment epithelium Bradykinin Transforming growth factor-β1 Extracellular matrix AbbreviationsBKBradykinin

CCK-8Cell counting Kit-8

ECMExtracellular matrix

ELISAenzyme-linked immunosorbent assay

FnFibronectin

KKSKallikrein-kinin system

KNGKininogen

LNLaminin

MMP-2Matrix metalloproteinase-2

ODOptical density

PBSPhosphate-buffered saline

PMSFPhenylmethylsulfonyl fluoride

PVRProliferative vitreoretinopathy

RIPARadio-immunoprecipitation assay

RPERetinal pigment epithelial

VNVitronectin

Download fulltext PDF



Autor: Wenting Cai - Qingquan Wei - Qingyu Liu - Chengda Ren - Junling Liu - Ruiling Zhang - Mengmei He - Qianyi Wang - Yaru Du

Fuente: https://link.springer.com/



DESCARGAR PDF




Documentos relacionados