Prevalence of feline haemoplasma in cats in DenmarkReportar como inadecuado




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Acta Veterinaria Scandinavica

, 58:78

First Online: 10 November 2016Received: 06 April 2016Accepted: 01 November 2016

Abstract

BackgroundInfections with the three feline haemotropic mycoplasmas Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis cause feline infectious anemia. The purpose of this study was to investigate the prevalence of carriage of feline haemoplasma in Danish cats in different age groups. The presence was detected by a conventional polymerase chain reaction PCR assay on blood samples as well as by real-time PCR RT-PCR.

ResultsThe study revealed a prevalence of 14.9% Candidatus Mycoplasma haemominutum positive cats and 1.5% Mycoplasma haemofelis positive cats. No cats were found positive for Candidatus Mycoplasma turicensis. The results showed a statistically significant higher prevalence in older >8 years cats compared to younger cats and a higher prevalence among domestic cats compared to purebred cats. As part of this study, we developed a cloning strategy to obtain Danish positive controls of haemoplasma 16S rRNA.

ConclusionFrom convenience-sampled cats in Denmark, we found that 16.4% were carriers of feline haemotropic mycoplasmas. Haemoplasma was mostly found in older and domestic cats. The prevalence found in Denmark is similar to that found in several other European countries.

KeywordsFeline haemoplasma Prevalence Real-time PCR Conventional PCR Risk factors Maja Benedicte Rosenqvist and Ann-Katrine Helene Meilstrup contributed equally to this work

Electronic supplementary materialThe online version of this article doi:10.1186-s13028-016-0260-1 contains supplementary material, which is available to authorized users.

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Autor: Maja Benedicte Rosenqvist - Ann-Katrine Helene Meilstrup - Jesper Larsen - John Elmerdahl Olsen - Asger Lundorff Jensen -

Fuente: https://link.springer.com/







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