Effects of lactoferrin derived peptides on simulants of biological warfare agentsReportar como inadecuado




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World Journal of Microbiology and Biotechnology

, 33:3

First Online: 10 November 2016Received: 14 September 2016Accepted: 03 November 2016

Abstract

Lactoferrin LF is an important immune protein in neutrophils and secretory fluids of mammals. Bovine LF bLF harbours two antimicrobial stretches, lactoferricin and lactoferampin, situated in close proximity in the N1 domain. To mimic these antimicrobial domain parts a chimeric peptide LFchimera has been constructed comprising parts of both stretches LFcin17–30 and LFampin265–284. To investigate the potency of this construct to combat a set of Gram positive and Gram negative bacteria which are regarded as simulants for biological warfare agents, the effect on bacterial killing, membrane permeability and membrane polarity were determined in comparison to the constituent peptides and the native bLF. Furthermore we aimed to increase the antimicrobial potency of the bLF derived peptides by cationic amino acid substitutions. Overall, the bactericidal activity of the peptides could be related to membrane disturbing effects, i.e. membrane permeabilization and depolarization. Those effects were most prominent for the LFchimera. Arginine residues were found to be crucial for displaying antimicrobial activity, as lysine to arginine substitutions resulted in an increased antimicrobial activity, affecting mostly LFampin265–284 whereas arginine to lysine substitutions resulted in a decreased bactericidal activity, predominantly in case of LFcin17–30.

KeywordsAntimicrobial activity Antimicrobial peptide Biowarfare simulants Lactoferrin LFchimera  Download fulltext PDF



Autor: Tjitske Sijbrandij - Antoon J. Ligtenberg - Kamran Nazmi - Enno C. I. Veerman - Jan G. M. Bolscher - Floris J. Bikker

Fuente: https://link.springer.com/







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