Potential effects of samsum ant, Brachyponera sennaarensis, venom on TNF-α-NF-κB mediated inflammation in CCL4-toxicity in vivoReportar como inadecuado




Potential effects of samsum ant, Brachyponera sennaarensis, venom on TNF-α-NF-κB mediated inflammation in CCL4-toxicity in vivo - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Lipids in Health and Disease

, 15:198

First Online: 18 November 2016Received: 20 May 2016Accepted: 08 November 2016

Abstract

BackgroundAnt venom shows antimicrobial, anti-parasitic and anti-inflammatory activities, both in vitro and in vivo. Our recent studies have confirmed the role of samsum ant venom SAV as a powerful antioxidant. This study aimed to investigate whether SAV as a potential treatment for CCl4-induced acute liver toxicity in an animal rat model.

MethodsThirty-two rats were assigned into four groups; the first one served as the control. The second group received a single dose of 1 ml-kg CCl4 in a 1:1 ratio with olive oil through an intraperitoneal injection. The third group received a single dose of 1 ml-kg CCl4 and then treated with SAV at a dose of 100 μg SAV twice a week for three weeks. The fourth group received a dose of 100 μg SAV only twice a week for three weeks. ELISA, RT-PCR and histopathological examinations were applied.

ResultsResults showed that antioxidant enzymes were significantly reduced in the diseased animals. SAV was found to significantly restore the oxidative stability in diseased animals. ELISA estimation and RT-PCR analysis also showed significant upregulation of both nuclear factor κB NF-κB and inhibitor κB IκB, respectively, in the diseased animals compared to the normal ones. The expression of tumour necrosis factor alpha TNF-α and pro-apoptotic receptor Fas were also significantly up-regulated in the diseased rats. Interestingly, SAV was found to significantly restore NF-κB, IκB and TNF-α in the diseased rats to the normal values. As a result, liver enzymes, serum proteins and lipid concentrations were significantly improved by SAV in CCl4-animals in comparison with the control ones. Moreover, SAV obviously improved the hepatic tissues of the same group was.

ConclusionSAV treatment restores the normal biochemical and oxidative stability by improving the TNF-α-NF-κB mediated inflammation in CCL4-treated rats.

KeywordsCCL4 Samsum ant venom Antioxidant activity NF-κB and IκB TNF-α AbbreviationsAkt1Protein kinase B

CATCatalase

DTNB5,5-dithiobis-2-nitrobenzoic acid

EDTAEthylenediaminetetraacetic acid

FASCell surface death receptor

GSHGlutathione

MDAMalondialdehyde

SAVSamsum ant venom

TBAThiobarbituric acid

TCATrichloroacetic acid

Download fulltext PDF



Autor: Jameel Al-Tamimi - Ibrahim M. Alhazza - Mohamed Al-Khalifa - Ali Metwalli - Ahmed Rady - Hossam Ebaid

Fuente: https://link.springer.com/







Documentos relacionados