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BMC Infectious Diseases

, 16:753

Bacterial and fungal diseases


BackgroundGroup A Streptococcus strains causing wide variety of diseases, recently became noticeable in eastern India, are not amenable to standard treatment protocol thus enhancing the possibility of disease morbidity by becoming antibiotic resistance.

MethodsThe association of Lancefield group A Streptococcal variation with degree of vir architectural diversity was evaluated using emm typing and restriction fragment length polymorphism analyses. The antibiotic sensitivity patterns were examined by modified Kirby-Bauer method of disk diffusion. Percentage calculations, 95% confidence interval and one-way ANOVA were used to assess differences in proportions.

ResultsOur observations revealed 20 different emm types and 13 different HaeIII vir typing patterns. A 1.2 kb fragment was found in all HaeIII typing pattern. Fragments of 1.2 kb and 550 bp were conserved in majority of the isolates. HinfI digestion was found proficient in differentiating the strains of same vir typing patterns. Strong predominance of speC 85% and speF 80% genes have been observed encoding exotoxins production. 4 isolates were found to be erythromycin resistant and were of genotype emm49. High degree of tetracycline resistance was shown by 53.57% isolates which belonged to 12 different emm genotypes.

ConclusionsThese findings suggested that in addition to emm typing, sequential application of HaeIII and HinfI restriction enzymes in vir typing analysis is an effective tool for group A streptococcal molecular characterization associated with antibiotic resistance.

KeywordsStreptococcus pyogenes GAS emm typing vir typing Virulence regulon Restriction enzyme digestion Antibiotic sensitivity patterns Exotoxin gene AbbreviationsAGNAcute glomerulonephritis

ARFAcute rheumatic fever

BHI brothBrain heart infusion broth

CLSIClinical and Laboratory Standards Institute

E.N.T. OPDEar nose and throat outpatient department

GASGroupA Streptococcus

PCRPolymerase chain reaction

RFLPRestriction fragment length polymorphism

RHDRheumatic heart disease

S. pyogenesStreptococcus pyogenes

Electronic supplementary materialThe online version of this article doi:10.1186-s12879-016-2079-9 contains supplementary material, which is available to authorized users.

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Autor: Dipanwita Ray - Somnath Saha - Sukanta Sinha - Nishith Kumar Pal - Basudev Bhattacharya


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