Biodistribution of a High Dose of Diamond, Graphite, and Graphene Oxide Nanoparticles After Multiple Intraperitoneal Injections in RatsReportar como inadecuado

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Nanoscale Research Letters

, 10:398

First Online: 12 October 2015Received: 20 June 2015Accepted: 07 October 2015


Carbon nanoparticles have recently drawn intense attention in biomedical applications. Hence, there is a need for further in vivo investigations of their biocompatibility and biodistribution via various exposure routes. We hypothesized that intraperitoneally injected diamond, graphite, and graphene oxide nanoparticles may have different biodistribution and exert different effects on the intact organism. Forty Wistar rats were divided into four groups: the control and treated with nanoparticles by intraperitoneal injection 4 mg of nanoparticles-kg body weight eight times during the 4-week period. Blood was collected for evaluation of blood morphology and biochemistry parameters. Photographs of the general appearance of each rat’s interior were taken immediately after sacrifice. The organs were excised and their macroscopic structure was visualized using a stereomicroscope. The nanoparticles were retained in the body, mostly as agglomerates. The largest agglomerates up to 10 mm in diameter were seen in the proximity of the injection place in the stomach serous membrane, between the connective tissues of the abdominal skin, muscles, and peritoneum. Numerous smaller, spherical-shaped aggregates diameter around 2 mm were lodged among the mesentery. Moreover, in the connective and lipid tissue in the proximity of the liver and spleen serosa, small aggregates of graphite and graphene oxide nanoparticles were observed. However, all tested nanoparticles did not affect health and growth of rats. The nanoparticles had no toxic effects on blood parameters and growth of rats, suggesting their potential applicability as remedies or in drug delivery systems.

KeywordsNanoparticles Diamond Graphite Graphene oxide Rat Biodistribution Vascular system AbbreviationsALPAlkaline phosphatase

ALTAlanine aminotransferase

ASTAspartate aminotransferase

BUNBlood urea nitrogen

BWBody weight

CNPCarbon nanoparticles

DNDiamond nanoparticles

GNGraphite nanoparticles

GOGraphene oxide

LDHLactate dehydrogenase

MPSMononuclear phagocyte system

MWCNTMulti-walled carbon nanotubes

NDNanodiamond particles

TEMTransmission electron microscopy


TPTotal protein

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Autor: Natalia Kurantowicz - Barbara Strojny - Ewa Sawosz - Sławomir Jaworski - Marta Kutwin - Marta Grodzik - Mateusz Wierzbicki


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