Trypanosome infection rates in tsetse flies in the -silent- sleeping sickness focus of Bafia in the Centre Region in CameroonReport as inadecuate

Trypanosome infection rates in tsetse flies in the -silent- sleeping sickness focus of Bafia in the Centre Region in Cameroon - Download this document for free, or read online. Document in PDF available to download.

Parasites and Vectors

, 8:528

First Online: 12 October 2015Received: 01 August 2015Accepted: 07 October 2015


BackgroundThe Bafia sleeping sickness focus of Cameroon is considered as -silent- with no case reported for about 20 years despite medical surveys performed during the last decades. In this focus, all epidemiological factors that can contribute to trypanosomes transmission are present. To update our knowledge on the current risks of Human and Animal African trypanosomiases, different trypanosome species were identified in midguts of tsetse flies captured in the Bafia focus.

MethodsTsetse flies were trapped using pyramidal traps. Each tsetse fly was identified and live flies were dissected and their midguts collected. DNA was extracted from each midgut and thereafter, blood meals and different trypanosome species were identified with molecular tools. The biological data were transported onto maps in order to have their distribution.

ResultsOf the 98 traps set up, 461 Glossina palpalis palpalis were captured; 322 69.8 % tsetse flies were dissected and 49 15.2 % teneral flies identified. The average apparent density of tsetse flies per day was 1.18. Of the 35 10.9 % blood meals collected, 82 % were taken on pigs and 17.6 % on humans. Eighty two 25.5 % trypanosome infections were identified: 56 17.4 % T. congolense savannah, 17 5.3 % T. congolense forest, 5 1.6 % T. vivax and 4 1.2 % T. brucei s.l. No infection of T. simiae and T. b. gambiense was identified. Sixty seven 81.7 % infections were single and 15 18.3 % mixed involving one triple infection T. congolense forest, T. brucei and T. vivax and 14 double infections: 11 T. congolense forest and T. congolense savannah, two T. congolense savannah and T. brucei, and one of T. brucei and T. vivax. The generated maps show the distribution of tsetse flies and trypanosome infections across the focus.

ConclusionThis study has shown that animal trypanosomes remain an important problem in this region. Meanwhile, it is very likely that HAT does not seem anymore to be a public health problem in this focus. The generated maps enabled us to define high risk transmission areas for AAT, and where disease control must be focused in order to improve animal health as well as the quantity of animal proteins.

KeywordsSleeping sickness African animal trypanosomiasis Trypanosomes Glossina palpalis palpalis Mid-guts  Download fulltext PDF

Author: Gustave Simo - Pierre Fongho - Oumarou Farikou - Prosper Innocent Ndjeuto Ndjeuto-Tchouli - Judith Tchouomene-Labou - Flobe


Related documents