Protective effects of extracts from Pomegranate peels and seeds on liver fibrosis induced by carbon tetrachloride in ratsReportar como inadecuado




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BMC Complementary and Alternative Medicine

, 15:389

First Online: 27 October 2015Received: 17 March 2015Accepted: 14 October 2015

Abstract

BackgroundLiver fibrosis is a feature in the majority of chronic liver diseases and oxidative stress is considered to be its main pathogenic mechanism. Antioxidants including vitamin E, are effective in preventing liver fibrogenesis. Several plant-drived antioxidants, such as silymarin, baicalin, beicalein, quercetin, apigenin, were shown to interfere with liver fibrogenesis. The antioxidans above are polyphenols, flavonoids or structurally related compounds which are the main chemical components of Pomegranate peels and seeds, and the antioxidant activity of Pomegranate peels and seeds have been verified. Here we investigated whether the extracts of pomegranate peels EPP and seeds EPS have preventive efficacy on liver fibrosis induced by carbon tetrachloride CCl4 in rats and explored its possible mechanisms.

MethodsThe animal model was established by injection with 50 % CCl4 subcutaneously in male wistar rats twice a week for four weeks. Meanwhile, EPP and EPS were administered orally every day for 4 weeks, respectively. The protective effects of EPP and EPS on biochemical metabolic parameters, liver function, oxidative markers, activities of antioxidant enzymes and liver fibrosis were determined in CCl4-induced liver toxicity in rats.

ResultsCompared with the sham group, the liver function was worse in CCl4 group, manifested as increased levels of serum alanine aminotransferase, aspartate aminotransferase and total bilirubin. EPP and EPS treatment significantly ameliorated these effects of CCl4. EPP and EPS attenuated CCl4–induced increase in the levels of TGF-β1, hydroxyproline, hyaluronic acid laminin and procollagen type III. They also restored the decreased superoxide dismutase SOD, glutathione peroxidase GSH-Px activities and inhibited the formation of lipid peroxidized products in rats treated with CCl4.

ConclusionThe EPP and EPS have protective effects against liver fibrosis induced by CCl4, and its mechanisms might be associated with their antioxidant activity, the ability of decreasing the level of TGF-β1 and inhibition of collagen synthesis.

KeywordsThe extract of pomegranate peels The extract of pomegranate seeds Liver fibrosis Oxidative stress  Download fulltext PDF



Autor: Xiang-lan Wei - Ru-tang Fang - Yong-hua Yang - Xue-yuan Bi - Guo-xia Ren - A-li Luo - Ming Zhao - Wei-jin Zang

Fuente: https://link.springer.com/







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