Immunomodulatory activity of Tityus serrulatus scorpion venom on human T lymphocytesReport as inadecuate

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Journal of Venomous Animals and Toxins including Tropical Diseases

, 21:46

Animal toxins: Exploring novel bioactive compounds from toads, snakes and scorpions


BackgroundTityus serrulatus scorpion venom TsV contains toxins that act on K and Na channels and account for the venom’s toxic effects. TsV can activate murine peritoneal macrophages, but its effects on human lymphocytes have been poorly investigated. Considering that lymphocytes may play an important role in envenomation, we assessed whether TsV affects the expression of phenotypic CD3, CD4, and CD8 and activation CD69, CD25, and HLA-DR markers, cell proliferation, and cytokine production in peripheral blood mononuclear cells.

MethodsCytotoxicity of TsV was evaluated via the MTT assay. Cell proliferation, expression of phenotypic and activation markers, and release of cytokines were assessed using flow cytometry, after treatment with non-cytotoxic concentrations of TsV. The combined use of carboxyfluorescein diacetate succinimidyl ester and monoclonal antibodies against phenotypic and activation markers enabled us to simultaneously assess cell proliferation extent and cell activation status, and to discriminate among cell subpopulations.

ResultsTsV at concentrations of 25 to 100 μg-mL were not cytotoxic towards peripheral blood mononuclear cells. TsV did not induce significant changes in lymphocyte subpopulations or in the expression of activation markers on CD4 and CD8 T cells. TsV inhibited the phytohemagglutinin-stimulated lymphocyte proliferation, particularly in the CD8 CD25 T lymphocyte subset. TsV alone, at 50 and 100 μg-mL, did not induce peripheral blood mononuclear cell proliferation, but elicited the production and release of IL-6, a proinflammatory cytokine that plays an important role in innate and adaptive immune responses.

ConclusionsTsV is a potential source of molecules with immunomodulatory action on human T lymphocytes.

KeywordsImmunomodulation Immunophenotyping T lymphocyte Cell proliferation Cytokine Tityus serrulatus venom Electronic supplementary materialThe online version of this article doi:10.1186-s40409-015-0046-3 contains supplementary material, which is available to authorized users.

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Author: Andrea Casella-Martins - Lorena R Ayres - Sandra M Burin - Fabiana R Morais - Juliana C Pereira - Lucia H Faccioli - Su


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