Low dose triterpene-quinone fraction from Ardisia crispa root precludes chemical-induced mouse skin tumor promotionReportar como inadecuado




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BMC Complementary and Alternative Medicine

, 15:431

First Online: 05 December 2015Received: 20 May 2015Accepted: 01 December 2015

Abstract

BackgroundDrastic increment of skin cancer incidence has driven natural product-based chemoprevention as a promising approach in anticancer drug development. Apart from its traditional usages against various ailments, Ardisia crispa Family: Myrsinaceae specifically its triterpene-quinone fraction TQF which was isolated from the root hexane extract ACRH was recently reported to exert antitumor promoting activity in vitro. This study aimed at determining chemopreventive effect of TQF against chemically-induced mouse skin tumorigenesis as well as elucidating its possible pathways.

MethodsMice n = 10 were initiated with single dose of 7,12-dimethylbenzαanthracene DMBA 390 nmol-100 μl followed by, a week later, repeated promotion twice weekly; 20 weeks with 12-O-tetradecanoylphorbol-13-acetate TPA 1.7 nmol-100 μl. TQF 10, 30 and 100 mg-kg and curcumin 10 mg-kg; reference were, respectively, applied topically to DMBA-TPA-induced mice 30 min before each TPA application. Upon termination, histopathological and biochemical analysis, as well as Terminal deoxynucleotidyl transferase dUTP nick end labeling TUNEL and transcription factor enzyme-linked immunosorbent assay ELISA assays were performed to elucidate the potential mechanism of TQF.

ResultsWith comparison to the carcinogen control, results revealed that lower dose of TQF 10 mg-kg conferred antitumor promoting effect via significant P < 0.05 suppression against lipid peroxidation LPO, apoptotic index cell death and nuclear factor-kappa B NF-κB, along with reduction of keratinocyte proliferation; whilst its higher dose 100 mg-kg was found to promote tumorigenesis by significantly P < 0.05 increasing LPO and apoptotic index, in addition to aggravating keratinocyte proliferation.

ConclusionsThis study evidenced that TQF, particularly at its lower dosage 10 mg-kg, ameliorated DMBA-TPA-induced mouse skin tumorigenesis. Though, future investigations are warranted to determine the lowest possible therapeutic dose of TQF in subsequent in vivo chemopreventive studies.

KeywordsArdisia crispa Anti-tumor promoting DMBA-TPA-induced skin tumorigenesis Apoptotic index NF-KappaB Looi Ting Yeong, Latifah Saiful Yazan, Huzwah Khaza’ai and Norhafizah Mohtarrudin contributed equally to this work.

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Autor: Looi Ting Yeong - Roslida Abdul Hamid - Latifah Saiful Yazan - Huzwah Khaza’ai - Norhafizah Mohtarrudin

Fuente: https://link.springer.com/



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