Quantification of the CBD-FITC conjugates surface coating on cellulose fibresReportar como inadecuado




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1 IBB - Institute for Biotechnology and Bioengineering 2 3ICBAS - Instituto de Ciências Biomédicas de Abel Salazar 3 CERMAV - Centre de recherches sur les macromolécules végétales

Abstract : Background: Cellulose Binding Domains CBD were conjugated with fluorescein isothiocyanate FITC. The surface concentration of the Binding Domains adsorbed on cellulose fibres was determined by fluorescence image analysis. Results: For a CBD-FITC concentration of 60 mg-L, a coating fraction of 78% and 110% was estimated for Portucel and Whatman fibres, respectively. For a saturating CBD concentration, using Whatman CF11 fibres, a surface concentration of 25.2 × 10-13 mol-mm2 was estimated, the equivalent to 4 protein monolayers. This result does not imply the existence of several adsorbed protein layers. Conclusion: It was verified that CBDs were able to penetrate the fibres, according to confocal microscopy and TEM-immunolabelling analysis. The surface concentration of adsorbed CBDs was greater on amorphous fibres phosphoric acid swollen than on more crystalline ones Whatman CF11 and Sigmacell 20.





Autor: R. Pinto - A.L. Amaral - E.C. Ferreira - M. Mota - M. Vilanova - K. Ruel - M. Gama -

Fuente: https://hal.archives-ouvertes.fr/



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