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Journal of Animal Science and Biotechnology

, 6:55

First Online: 22 December 2015Received: 07 April 2015Accepted: 09 December 2015

Abstract

BackgroundIdentification of genetic variants, including SNPs Single Nucleotide Polymorphisms, CNVs Copy Number Variations and alternative splicing, within functional genes has received increasing attention in animal science research. HGF Hepatocyte Growth Factor is a very important growth factor that works as a mitogen or a morphogen during tissue growth, development and regeneration. However, to date, the functions of genetic variants within the bovine HGF gene, particularly their effects on mRNA expression, have not been determined well.

ResultsThe present study aimed to perform association analysis between genetic variants and mRNA expression for the bovine HGF gene in Qinchuan cattle using various strategies, including PCR-RFLP Restriction Fragment Length Polymorphism, qPCR Quantitative Real-time quantitative PCR, TA cloning, DNA sequencing and bioinformatics analysis. A total of five SNPs were identified and only SV1 locus significantly affected HGF mRNA expression in fetal skeletal muscle P < 0.05. Heterozygous genotype individuals showed significantly higher HGF expression P < 0.05, which was significantly greater in the -CTCCAGGGTT- combined genotype than that in the -CCCCGGGGTT- combined genotype P < 0.05. In addition, two alternative splicing variations, HGF-W and HGF-M, were identified, which resulted from alternative 3′ splice sites of exon 5, and HGF-W showed higher mRNA levels than HGF-M in all tissues.

ConclusionIn summary, genetic variations within the HGF gene affected mRNA expression. These findings provide new insight into the molecular characteristics and functions of bovine HGF.

KeywordsAlternative splicing Expression HGF Qinchuan cattle SNPs AbbreviationsANOVAone-way analysis of variance

CNVsCopy number variations

HGFHepatocyte Growth Factor

MAPKmitogen activated protein kinase

PI3Kphosphatidy linositol 3-kinase

qPCRQuantitative Real-time quantitative PCR

SNPsSingle Nucleotide Polymorphisms

STATsignal transducer and activator of transcription

UTRuntranslated region

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Autor: Hanfang Cai - Yang Zhou - Wenchao Jia - Bowen Zhang - Xianyong Lan - Chuzhao Lei - Xintang Fang - Hong Chen

Fuente: https://link.springer.com/



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