Molecular cloning and expression patterns of the cholesterol side chain cleavage enzyme CYP11A1 gene during the reproductive cycle in goose Anas cygnoidesReportar como inadecuado




Molecular cloning and expression patterns of the cholesterol side chain cleavage enzyme CYP11A1 gene during the reproductive cycle in goose Anas cygnoides - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Animal Science and Biotechnology

, 6:54

First Online: 22 December 2015Received: 31 July 2015Accepted: 01 December 2015

Abstract

BackgroundCYP11A1, a gene belonging to the family 11 of cytochrome P450, encodes a crucial steroidogenic enzyme that catalyzes the initial step in the production of all classes of steroids. Many studies show that CYP11A1 plays a role in ovary function. However, the role of CYP11A1 in goose reproductive cycle remains largely unknown.

ResultsIn this study, full-length CYP11A1 cDNA of Zhedong goose was obtained using reverse transcription polymerase chain reaction RT-PCR and rapid amplification of cDNA ends RACE. The cDNA consisted of a 96-base pair bp 5′untranslated region UTR, a 179-bp 3′UTR and a 1509-bp open reading frame. The open reading frame encodes a putative 503 amino acid protein that shares high homology with CYP11A1 of other birds. The amino acid sequence possesses conserved domains of the P450 superfamily, which include the steroid-binding domain and the heme-binding region. Real-time quantitative polymerase chain reaction qPCR analysis revealed CYP11A1 mRNA was expressed ubiquitously in every Zhedong goose tissue analyzed, including the heart, liver, glandular stomach, lung, spleen, kidney, intestinum tenue, intestinum crassum, cerebrum, cerebellum, muscle, oviduct, pituitary, hypothalamus and ovary

The relatively low levels of CYP11A1 mRNA were detected in pituitary, ovary and oviduct tissues at ovulation when compared with levels at oviposition. Interestingly, higher expression was observed in ovary and oviduct tissues during brooding. Lastly, higher mRNA expression of Yangzhou geese was detected during the ovulation period than that of Zhedong geese.

ConclusionsOur findings reveal the sequence characterization and expression patterns of the CYP11A1 gene during the goose reproductive cycle, which may provides correlative evidence that CYP11A1 expression is important in reproduction activity.

KeywordsCYP11A1 Gene expression Goose Reproduction AbbreviationsP450sccside-chain cleavage cytochrome P450

CYP11A1cholesterol side chain cleavage enzyme

RT-PCRreverse transcription polymerase chain reaction

RACErapid amplification of cDNA ends

bpbase pair

UTRuntranslated region

qPCRquantitative polymerase chain reaction

GAPDHglyceraldehyde-3-phosphate dehydrogenase

HPGhypothalamus-pituitary-gonad

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Autor: Qi Xu - Yadong Song - Yang Chen - Ran Liu - Yang Zhang - Yang Li - Zhengyang Huang - Wenming Zhao - Guobin Chang - Guohon

Fuente: https://link.springer.com/







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