Production of cyathane type secondary metabolites by submerged cultures of Hericium erinaceus and evaluation of their antibacterial activity by direct bioautographyReportar como inadecuado




Production of cyathane type secondary metabolites by submerged cultures of Hericium erinaceus and evaluation of their antibacterial activity by direct bioautography - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Fungal Biology and Biotechnology

, 2:8

First Online: 22 December 2015Received: 17 November 2015Accepted: 09 December 2015

Abstract

BackgroundFungi of the phylum Basidiomycota are well-known to form a broad spectrum of biologically active secondary metabolites, especially low molecular weight compounds such as terpenoids. Hericium erinaceus produces various cyathane type diterpenoids including erinacines. However, no quantitative data and production kinetics have been reported on the biosynthesis of the erinacines C and P in submerged cultures. In the present study, the production of erinacine C was optimized, and the product formation kinetics as well as the antimicrobial activity were studied by high-performance liquid chromatography HPLC, high-performance thin-layer chromatography HPTLC and direct bioautography.

ResultsOatmeal and Edamin K were identified to be crucial media components for an efficient production of erinacine C. The highest concentrations of erinacine C were obtained in the optimized culture medium on the 9 culture day approximately 260 mg L. The production of erinacine P was strongly time dependent. The maximum concentration of erinacine P of 184 mg L was observed on the third culture day. Afterwards, the concentrations of erinacine P decreased while the concentrations of erinacine C steadily increased. Comparable results were obtained by HPTLC with UV detection and HPLC with diode-array detection DAD analyses. Direct bioautography allowed for an additional analysis of the antimicrobial activity of the secondary metabolites.

ConclusionsThe C and N sources oatmeal and Edamin K induced the formation of erinacine C. Detailed product formation kinetics of the erinacines C and P have been reported for the first time. HPTLC combined with the Aliivibrio fischeri bioassay allowed for an instant detection of cyathane diterpenoids in crude extracts and for an evaluation of the antimicrobial activity of the secondary metabolites directly on the plate.

KeywordsHericium erinaceus Cyathane type diterpenoids Erinacine C Erinacine P HPTLC TLC-MS interface Aliivibrio fischeri Electronic supplementary materialThe online version of this article doi:10.1186-s40694-015-0018-y contains supplementary material, which is available to authorized users.

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Autor: T. Shen - G. Morlock - H. Zorn

Fuente: https://link.springer.com/







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