Meloidogyne incognita - rice Oryza sativa interaction: a new model system to study plant-root-knot nematode interactions in monocotyledonsReportar como inadecuado

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, 7:23

First Online: 22 September 2014Received: 22 December 2013Accepted: 28 August 2014


BackgroundPlant-parasitic nematodes developed strategies to invade and colonize their host plants, including expression of immune suppressors to overcome host defenses. Meloidogyne graminicola and M. incognita are root-knot nematode RKN species reported to damage rice Oryza sativa L. cultivated in upland and irrigated systems. Despite M. incognita wide host range, study of the molecular plant - RKN interaction has been so far limited to a few dicotyledonous model plants. The aim of this study was to investigate if the rice cv. Nipponbare widely used in rice genomic studies could be used as a suitable monocotyledon host plant for studying M. incognita pathogenicity mechanisms. Here we compared the ability of M. graminicola and M. incognita to develop and reproduce in Nipponbare roots. Next, we tested if RKNs modulates rice immunity-related genes expression in galls during infection and express the Mi-crt gene encoding an immune suppressor.

ResultsRoot galling, mature females, eggs and newly formed J2s nematodes were obtained for both species in rice cultivated in hydroponic culture system after 4-5 weeks. Meloidogyne graminicola reproduced at higher rates than M. incognita on Nipponbare and the timing of infection was shorter. In contrast, the infection characteristics compared by histological analysis were similar for both nematode species. Giant cells formed from 2 days after infection DAI with M. graminicola and from 6 DAI with M. incognita. Real-time PCR qRT-PCR data indicated that RKNs are able to suppress transcription of immune regulators genes, such as OsEDS1, OsPAD4 and OsWRKY13 in young galls. Four M. incognita reference genes Mi-eif-3, Mi-GDP-2, Mi-Y45F10D.4, and Mi-actin were selected for normalizing nematode gene expression studies in planta and in pre-parasitic J2s. Meloidogyne incognita expressed the immune suppressor calreticulin gene Mi-crt in rice roots all along its infection cycle.

ConclusionRKNs repress the transcription of key immune regulators in rice, likely in order to lower basal defence in newly-formed galls. The calreticulin Mi-CRT can be one of the immune-modulator effectors secreted by M. incognita in rice root tissues. Together, these data show that rice is a well suited model system to study host- M. incognita molecular interactions in monocotyledons.

KeywordsRice Root-knot nematodes Giant cells Immunity suppression Meloidogyne graminicola Meloidogyne incognita AbbreviationsRKNRoot-knot nematode

DAIDays after inoculation

GCsGiant cells

NCsNeighboring giant cells

NFSNematode feeding site

J2Juvenile stage 2

CqCycle quantification

SASalicylic acid

JAJasmonic acid



SARSA-dependent systemic acquired resistance

MAPKMitogen-activated protein kinase

PTIPAMP-triggered immunity

PAMPsPathogen-associated molecular patterns

ETIEffector-triggered immunity

Electronic supplementary materialThe online version of this article doi:10.1186-s12284-014-0023-4 contains supplementary material, which is available to authorized users.

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Autor: Phong Vũ Nguyễn - Stéphane Bellafiore - Anne-Sophie Petitot - Rana Haidar - Aurélie Bak - Amina Abed - Pascal Gantet


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