Prevalence and phylogenetic analysis of tick-borne encephalitis virus TBEV in field-collected ticks Ixodes ricinus in southern SwitzerlandReportar como inadecuado




Prevalence and phylogenetic analysis of tick-borne encephalitis virus TBEV in field-collected ticks Ixodes ricinus in southern Switzerland - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Parasites and Vectors

, 7:443

First Online: 22 September 2014Received: 06 May 2014Accepted: 13 September 2014

Abstract

BackgroundTick-borne encephalitis is the most common tick-borne viral infection in Europe with 3,000 human cases reported each year. In Western Europe, the castor bean tick, Ixodes ricinus, is the principal vector of the tick-borne encephalitis virus TBEV. TBEV appears to be spreading geographically and was recently detected for the first time in Canton Valais in the southern part of Switzerland. The purpose of the present study was to survey the I. ricinus tick populations of Canton Valais for TBEV.

MethodsWe collected a total of 19,331 I. ricinus ticks at 45 different sites in Canton Valais between 2010 and 2013. Ticks were processed in pools and tested for TBEV using reverse transcription quantitative PCR. The NS5 gene and the envelope gene of the TBEV isolates were partially sequenced for phylogenetic analysis.

ResultsTBEV was detected in tick populations at six of the 45 sites. These six sites were all located in a 33 km transect along the Rhône River. TBEV was detected in two sites for three of the four years of the study showing the temporal persistence of the pathogen. Prevalence of TBEV in the six positive sites ranged from 0.16% to 11.11%. Phylogenetic analysis found that all TBEV isolates from Canton Valais belonged to the European subtype. Genetic analysis found two distinct lineages of TBEV suggesting that Canton Valais experienced two independent colonization events.

ConclusionsTBEV appears to be well established at certain locations in Canton Valais.

KeywordsEnvelope protein Flavivirus Ixodes ricinus Switzerland Tick Tick-borne encephalitis virus Tick-borne disease Electronic supplementary materialThe online version of this article doi:10.1186-1756-3305-7-443 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Nadia Rieille - Stéphane Bressanelli - Caio C M Freire - Séverine Arcioni - Lise Gern - Olivier Péter - Maarten J Voo

Fuente: https://link.springer.com/







Documentos relacionados