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BMC Veterinary Research

, 10:216

Neurology and neuroscience


BackgroundAnatomical differences between humans and domestic mammals preclude the use of reported stereotactic approaches to the brainstem in animals. In animals, brainstem biopsies are required both for histopathological diagnosis of neurological disorders and for research purposes. Sheep are used as a translational model for various types of brain disease and therefore a species-specific approach needs to be developed. The aim of the present study was to establish a minimally invasive, accurate and reproducible stereotactic approach to the brainstem of sheep, using the magnetic resonance imaging guided Brainsight frameless stereotactic system.

ResultsA transoccipital transcerebellar approach with an entry point in the occipital bone above the vermis between the transverse sinus and the external occipital protuberance was chosen. This approach provided access to the target site in all heads. The overall mean needle placement error was 1.85 ± 1.22 mm.

ConclusionsThe developed transoccipital transcerebellar route is short, provides accurate access to the ovine caudal cranial fossa and is a promising approach to be further assessed in live animals.

KeywordsSheep Brainstem Stereotaxy Transcerebellar route Large animal model Electronic supplementary materialThe online version of this article doi:10.1186-s12917-014-0216-5 contains supplementary material, which is available to authorized users.

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Autor: Anne Staudacher - Anna Oevermann - Michael H Stoffel - Daniela Gorgas


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