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BMC Plant Biology

, 14:258

Genomics and evolution

Abstract

BackgroundNew SNP marker platforms offer the opportunity to investigate the relationships between wheat cultivars from different regions and assess the mechanism and processes that have led to adaptation to particular production environments. Wheat breeding has a long history in Kazakhstan and the aim of this study was to explore the relationship between key varieties from Kazakhstan and germplasm from breeding programs for other regions.

ResultsThe study revealed 5,898 polymorphic markers amongst ten cultivars, of which 2,730 were mapped in the consensus genetic map. Mapped SNP markers were distributed almost equally across the A and B genomes, with between 279 and 484 markers assigned to each chromosome. Marker coverage was approximately 10-fold lower in the D genome. There were 863 SNP markers identified as unique to specific cultivars, and clusters of these markers regions containing more than three closely mapped unique SNPs showed specific patterns on the consensus genetic map for each cultivar. Significant intra-varietal genetic polymorphism was identified in three cultivars Tzelinnaya 3C, Kazakhstanskaya rannespelaya and Kazakhstanskaya 15. Phylogenetic analysis based on inter-varietal polymorphism showed that the very old cultivar Erythrospermum 841 was the most genetically distinct from the other nine cultivars from Kazakhstan, falling in a clade together with the American cultivar Sonora and genotypes from Central and South Asia. The modern cultivar Kazakhstanskaya 19 also fell into a separate clade, together with the American cultivar Thatcher. The remaining eight cultivars shared a single sub-clade but were categorised into four clusters.

ConclusionThe accumulated data for SNP marker polymorphisms amongst bread wheat genotypes from Kazakhstan may be used for studying genetic diversity in bread wheat, with potential application for marker-assisted selection and the preparation of a set of genotype-specific markers.

KeywordsBread wheat Genetic polymorphism Clusters of unique markers Genetic phylogeny Kazakhstan Next-generation sequencing NGS Single nucleotide polymorphism SNP Unique markers AbbreviationsBACBacterial artificial chromosome

DPIDepartment of Primary Industries

ESTExpressed sequence tag

GBSGenotyping-by-sequencing

KASParCompetitive allele specific polymerase chain reaction

MASMarker-assisted selection, NGS, Next-generation sequencing

PICPolymorphism information content

SNPSingle nucleotide polymorphism

SSCPSingle strand confirmation polymorphism

SSRSimple sequence repeats

SRAPSequence related amplified polymorphism

Electronic supplementary materialThe online version of this article doi:10.1186-s12870-014-0258-7 contains supplementary material, which is available to authorized users.

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Autor: Yuri Shavrukov - Radoslaw Suchecki - Serik Eliby - Aigul Abugalieva - Serik Kenebayev - Peter Langridge

Fuente: https://link.springer.com/







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