Acute oral toxicity of Insampaedok-san, a traditional herbal formula, in rats and its protective effects against ovalbumin-induced asthma via anti-inflammatory and antioxidant propertiesReportar como inadecuado




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BMC Complementary and Alternative Medicine

, 14:365

Basic research

Abstract

BackgroundInsampaedok-san ren-shen-bai-du-san in Chinese is a traditional herbal formula widely used for the treatment of respiratory diseases in Korea and China. In this study, we investigated the acute oral toxicity of an Insampaedok-san water extract ISSE in rats and the antiasthmatic effects of ISSE and its mechanism in a model of asthma induced by ovalbumin OVA in mice.

MethodsIn a safety study, ISSE was administrated orally to rats of both sexes at single doses of 0 and 5000 mg-kg. We observed body weight changes, mortality, clinical signs, and gross pathological findings. In vitro antioxidant activity of ISSE was measured using 2,2-diphenyl-2-picrylhydrazyl and 2,2-azino-bis3-ethylbenzothiazoline-6-sulfonic acid radical scavenging methods. A model of asthma was established in mice by sensitization and challenge with OVA. We assessed the levels of type 2 T-helper cytokines, chemokines, and immunoglobulin levels, using enzyme-linked immunosorbent assays, and superoxide dismutase SOD activity using a kit.

ResultsNo adverse effects were observed in the acute ISSE toxicity study. ISSE showed potent free radical scavenging activity and inhibited the recruitment of inflammatory cells into the lung and mucus hypersecretion in OVA-challenged mice. ISSE significantly decreased levels of interleukin IL-4, IL-5, eotaxin, and OVA-specific immunoglobulin IgE, and increased SOD activity.

ConclusionsThese results indicate that ISSE is safe for human consumption and its antiasthmatic effect is associated with the ability of ISSE to attenuate inflammation and oxidative stress.

KeywordsInsampaedok-san Safety Cytokine Free radical scavenging Asthma Electronic supplementary materialThe online version of this article doi:10.1186-1472-6882-14-365 contains supplementary material, which is available to authorized users.

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Autor: Yeji Kim - Mee-Young Lee - Ohn-Soon Kim - Woo-Young Jeon - Hyeun-Kyoo Shin

Fuente: https://link.springer.com/



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