Bioanalytical characterisation of multiple endocrine- and dioxin-like activities in sediments from reference and impacted small rivers.Reportar como inadecuado




Bioanalytical characterisation of multiple endocrine- and dioxin-like activities in sediments from reference and impacted small rivers. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 INERIS - Institut National de l-Environnement Industriel et des Risques 2 DCMR - Laboratoire des mécanismes réactionnels 3 IRCM - Institut de recherche en cancérologie de Montpellier

Abstract : A comprehensive evaluation of organic contamination was performed in sediments sampled in two reference and three impacted small streams where endocrine disruptive ED effects in fish have been evidenced. The approach combined quantitative chemical analyses of more than 50 ED chemicals EDCs and a battery of in vitro bioassays allowing the quantification of receptor-mediated activities, namely estrogen ER, androgen AR, dioxin AhR and pregnane X PXR receptors. At the most impacted sites, chemical analyses showed the presence of natural estrogens, organochlorine pesticides, parabens, polycyclic aromatic hydrocarbons 16 PAHs, bisphenol A and alkylphenols, while synthetic steroids, myco-estrogens and phyto-estrogens were not detected. Determination of toxic-equivalent amounts showed that 28-96% of estrogenic activities in bioassays 0.2-6.3 ng-g 17beta-estradiol equivalents were explained by 17beta-estradiol and estrone. PAHs were major contributors 20-60% to the total dioxin-like activities. Interestingly, high PXR and antiAR activities were detected; however, the targeted analysed compounds could not explain the measured biological activities. This study highlighted the presence of multiple organic EDCs in French river sediments subjected to mixed diffuse pollution, and argues for the need to further identify AR and PXR active compounds in the aquatic environment.





Autor: Said Kinani - Stéphane Bouchonnet - Nicolas Creusot - Sophie Bourcier - Patrick Balaguer - Jean-Marc Porcher - Sélim Aït-Aïss

Fuente: https://hal.archives-ouvertes.fr/



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