Effects of KH-204 on the expression of heat shock protein 70 and germ cell apoptosis in infertility rat modelsReportar como inadecuado




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BMC Complementary and Alternative Medicine

, 14:367

Basic research

Abstract

BackgroundIdiopathic infertility is a significant number of causes of male infertility. Empirical treatments are used for idiopathic male infertility, and antioxidant supplementation is a kind of management of oxidative stress related infertility. We investigated the antioxidant effects of the modified Ojayeonjonghwan KH-204 in a rat model of cryptorchidism.

MethodMale rats were divided into four groups n = 8 in each: a normal control group, a cryptorchidism-induced control group and two cryptorchidism-induced groups treated p.o. with either 200 or 400 mg-kg, KH-204 for 4 weeks. The testes and epididymides from rats in all groups were removed, weighed and subjected to histological examination and semen analysis after surgery. Oxidative stress was assessed by measuring 8-hydroxy-20-deoxyguanosine 8-OHdG, superoxide dismutase SOD and heat shock protein HSP levels. Apoptosis was determined using a terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling assay.

ResultsTreatment with the multi-herbal medicine KH-204 1 increased the mean weight of the cryptorchid testes; 2 restored sperm counts, motility and germinal cell layer thickness; 3 decreased levels of 8-OHdG and increased levels of SOD; and 4 decreased HSP70 levels and apoptosis.

ConclusionsKH-204 reduces the oxidative stress in an experimental rat model of cryptorchidism, and it may alleviate HSP expression and germ cell apoptosis.

KeywordsInfertility, male Phytotherapy Spermatozoa Antioxidants Electronic supplementary materialThe online version of this article doi:10.1186-1472-6882-14-367 contains supplementary material, which is available to authorized users.

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Autor: Woong Jin Bae - U Syn Ha - Kang Sup Kim - Su Jin Kim - Hyuk Jin Cho - Sung Hoo Hong - Ji Youl Lee - Zhiping Wang -

Fuente: https://link.springer.com/







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