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Journal of Natural Medicines

, Volume 68, Issue 4, pp 677–685

First Online: 22 June 2014Received: 27 January 2014Accepted: 27 April 2014

Abstract

In May 2011, numerous poppy plants closely resembling Papaver bracteatum Lindl., a type of narcotic plant that is illegal in Japan, were distributed directly from several large flower shops or through online shopping throughout Japan, including the Tokyo Metropolitan area. In order to better identify the narcotic plants, the relative nuclear DNA content at the vegetative stage was measured by flow cytometric FCM analysis in 3 closely-related species of the genus Papaver section Oxytona, namely P. orientale, P. pseudo-orientale, and P. bracteatum, based on the difference between the chromosome numbers of these species. The results showed that the nuclear DNA content differed between these 3 species, and that most of the commercially distributed plants examined in this study could be identified as P. bracteatum. The remaining plants were P. pseudo-orientale, a non-narcotic plant. In addition, the FCM results for the identification of P. bracteatum completely agreed with the results obtained by the morphological analysis, the inter-genic spacer sequence of rpl16–rpl14 PS-ID sequence of chloroplast DNA, and the presence of thebaine. These results clearly indicate the usefulness of FCM analysis for the identification of P. bracteatum plants, including when they are in their vegetative stage.

KeywordsPapaver bracteatum Oxytona section DNA content Flow cytometry Identification of species Morphological characteristics PS-ID sequence Thebaine  Download fulltext PDF



Autor: Masako Aragane - Daisuke Watanabe - Jun’ichi Nakajima - Masao Yoshida - Masao Yoshizawa - Tomohiro Abe - Rei Nishiyama -

Fuente: https://link.springer.com/



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