Crystal Structure of Talaromyces cellulolyticus Formerly Known as Acremonium cellulolyticus GH Family 11 XylanaseReportar como inadecuado




Crystal Structure of Talaromyces cellulolyticus Formerly Known as Acremonium cellulolyticus GH Family 11 Xylanase - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Applied Biochemistry and Biotechnology

, Volume 174, Issue 4, pp 1599–1612

First Online: 20 August 2014Received: 07 May 2014Accepted: 29 July 2014

Abstract

Talaromyces cellulolyticus formerly known as Acremonium cellulolyticus is one of the mesophilic fungi that can produce high levels of cellulose-related enzymes and are expected to be used for the degradation of polysaccharide biomass. In silico analysis of the genome sequence of T. cellulolyticus detected seven open reading frames ORFs showing homology to xylanases from glycoside hydrolase GH family 11. The gene encoding the GH11 xylanase C TcXylC with the highest activity was used for overproduction and purification of the recombinant enzyme, permitting solving of the crystal structure to a resolution of 1.98 Å. In the asymmetric unit, two kinds of the crystal structures of the xylanase were identified. The main structure of the protein showed a β-jelly roll structure. We hypothesize that one of the two structures represents the open form and the other shows the close form. The changing of the flexible region between the two structures is presumed to induce and accelerate the enzyme reaction. The specificity of xylanase toward the branched xylan is discussed in the context of this structural data and by comparison with the other published structures of xylanases.

KeywordsX-ray crystal structure Biomass Xylanase Hemicellulose Talaromyces cellulolyticus formerly known as Acremonium cellulolyticus β-Jelly roll  Download fulltext PDF



Autor: Misumi Kataoka - Fusamichi Akita - Yuka Maeno - Benchaporn Inoue - Hiroyuki Inoue - Kazuhiko Ishikawa

Fuente: https://link.springer.com/







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