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AMB Express

, 4:75

First Online: 10 October 2014Received: 04 August 2014Accepted: 23 September 2014

Abstract

Knowledge is scarce about the degradation of ketones in yeasts. For bacteria a subterminal degradation of alkanes to ketones and their further metabolization has been described which always involved Baeyer-Villiger monooxygenases BVMOs. In addition, the question has to be clarified whether alkenes are converted to ketones, in particular for the oil degrading yeast Candida maltosa little is known. In this study we show the degradation of the aliphatic ketone dodecane-2-one by Candida maltosa and the related yeasts Candida tropicalis, Candida catenulata and Candida albicans as well as Trichosporon asahii and Yarrowia lipolytica. One pathway is initiated by the formation of decyl acetate, resulting from a Baeyer-Villiger-oxidation of this ketone. Beyond this, an initial reduction to dodecane-2-ol by a keto reductase was clearly shown. In addition, two different ways to metabolize dodec-1-ene were proposed. One involved the formation of dodecane-2-one and the other one a conversion leading to carboxylic and dicarboxylic acids. Furthermore the induction of ketone degrading enzymes by dodecane-2-one and dodec-1-ene was shown. Interestingly, with dodecane no subterminal degradation products were detected and it did not induce any enzymes to convert dodecane-2-one.

KeywordsHydrocarbon alkene ketone Candida yeast biotransformation Electronic supplementary materialThe online version of this article doi:10.1186-s13568-014-0075-2 contains supplementary material, which is available to authorized users.

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Autor: Andy Beier - Veronika Hahn - Uwe T Bornscheuer - Frieder Schauer

Fuente: https://link.springer.com/







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