Initial stem cell adhesion on porous silicon surface: molecular architecture of actin cytoskeleton and filopodial growthReportar como inadecuado




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Nanoscale Research Letters

, 9:564

Porous Semiconductors - Science and Technology PSST2014

Abstract

The way cells explore their surrounding extracellular matrix ECM during development and migration is mediated by lamellipodia at their leading edge, acting as an actual motor pulling the cell forward. Lamellipodia are the primary area within the cell of actin microfilaments filopodia formation. In this work, we report on the use of porous silicon pSi scaffolds to mimic the ECM of mesenchymal stem cells from the dental pulp DPSC and breast cancer MCF-7 cells. Our atomic force microscopy AFM, fluorescence microscopy, and scanning electron microscopy SEM results show that pSi promoted the appearance of lateral filopodia protruding from the DPSC cell body and not only in the lamellipodia area. The formation of elongated lateral actin filaments suggests that pores provided the necessary anchorage points for protrusion growth. Although MCF-7 cells displayed a lower presence of organized actin network on both pSi and nonporous silicon, pSi stimulated the formation of extended cell protrusions.

KeywordsMesenchymal stem cells Cell adhesion Porosity Tissue engineering Electronic supplementary materialThe online version of this article doi:10.1186-1556-276X-9-564 contains supplementary material, which is available to authorized users.

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Autor: Pierre-Yves Collart-Dutilleul - Ivan Panayotov - Emilie Secret - Frédérique Cunin - Csilla Gergely - Frédéric Cuisinier

Fuente: https://link.springer.com/







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