Changes in various metabolic parameters in blood and milk during experimental Escherichia coli mastitis for primiparous Holstein dairy cows during early lactationReportar como inadecuado




Changes in various metabolic parameters in blood and milk during experimental Escherichia coli mastitis for primiparous Holstein dairy cows during early lactation - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Animal Science and Biotechnology

, 5:47

First Online: 17 October 2014Received: 28 May 2014Accepted: 10 October 2014

Abstract

BackgroundThe objective of this study was to characterize the changes in various metabolic parameters in blood and milk during IMI challenge with Escherichia coli E. coli for dairy cows during early lactation. Thirty, healthy primiparous Holstein cows were infused h = 0 with ~20-40 cfu of live E. coli into one front mammary quarter at ~4-6 wk in lactation. Daily feed intake and milk yield were recorded. At –12, 0, 3, 6, 12, 18, 24, 36, 48, 60, 72, 96, 108, 120, 132, 144, 156, 168, 180 and 192 h relative to challenge rectal temperatures were recorded and quarter foremilk was collected for analysis of shedding of E. coli. Composite milk samples were collected at -180 -132 -84 -36 -12, 12, 24, 36, 48, 60, 72, 84, 96, 132 and 180 h relative to challenge h = 0 and analyzed for lactate dehydrogenase LDH , somatic cell count, fat, protein, lactose, citrate, beta-hydroxybutyrate BHBA , free glucose fglu , and glucose-6-phosphate G6P . Blood was collected at -12, 0, 3, 6, 12, 18, 24, 36, 60, 72, 84, 132 and 180 h relative to challenge and analyzed for plasma non-esterified fatty acids NEFA , BHBA and glucose concentration. A generalized linear mixed model was used to determine the effect of IMI challenge on metabolic responses of cows during early lactation.

ResultsBy 12 h, E. coli was recovered from challenged quarters and shedding continued through 72 h. Rectal temperature peaked by 12 h post-challenge and returned to pre-challenge values by 36 h post-IMI challenge. Daily feed intake and milk yield decreased P <0.05 by 1 and 2 d, respectively, after mastitis challenge. Plasma BHBA decreased 12 h; P <0.05 from 0.96 ± 1.1 at 0 h to 0.57 ± 0.64 mmol-L by 18 h whereas concentration of plasma NEFA 18 h and glucose 24 h were significantly greater, 11 and 27%, respectively, after challenge. In milk, fglu, lactose, citrate, fat and protein yield were lower whereas yield of BHBA and G6P were higher after challenge when compared to pre-challenge values.

ConclusionsChanges in metabolites in blood and milk were most likely associated with drops in feed intake and milk yield. However, the early rise in plasma NEFA may also signify enhanced adipose tissue lipolysis. Lower concentrations of plasma BHBA may be attributed to an increase transfer into milk after IMI. Decreases in both milk lactose yield and % after challenge may be partly attributed to reduced conversion of fglu to lactose. Rises in G6P yield and concentration in milk after challenge 24 h may signify increased conversion of fglu to G6P. Results identify changes in various metabolic parameters in blood and milk after IMI challenge with E. coli in dairy cows that may partly explain the partitioning of nutrients and changes in milk components after IMI for cows during early lactation.

KeywordsCow Early lactation Escherichia coli Metabolism Electronic supplementary materialThe online version of this article doi:10.1186-2049-1891-5-47 contains supplementary material, which is available to authorized users.

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Autor: Kasey M Moyes - Torben Larsen - Peter Sørensen - Klaus L Ingvartsen

Fuente: https://link.springer.com/







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