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Annals of Clinical Microbiology and Antimicrobials

, 13:50

First Online: 07 November 2014Received: 14 May 2014Accepted: 17 October 2014

Abstract

BackgroundMethicillin-resistant Staphylococcus aureus MRSA are important pathogens in neonatal and pediatric intensive care units, which can cause severe infections in hospitalized children. Detection of the mec A gene and classification of the staphylococcal cassette chromosome mec SCCmec permit the characterization of MRSA strains isolated from infections caused by these microorganisms. In contrast, pulsed-field gel electrophoresis PFGE is used to type MRSA clones. This method is commonly used to analyze the epidemiology of bacteria causing nosocomial infections. The objective of this study was to detect and characterize MRSA isolated from clinical specimens of children hospitalized in the neonatal and pediatric intensive care units of the University Hospital of the Botucatu Medical School.

MethodsA total of 119SS. aureus strains were isolated from clinical specimens and the mec A gene was detected by PCR. SCCmec was detected by multiplex PCR and the clonal profile was analyzed by PFGE.

ResultsThe mec A gene was detected in 17.6% 21-119 of the isolates; 42.9% 9-21 of MRSA were characterized as SCCmec type III and 57.1% 12-21 as type IV. Analysis of the clonal profile of these strains revealed three distinct clones, with SCCmec type III being related to the Brazilian endemic clone and type IV to clones JCSC4469 and USA800.

ConclusionsReplacement of clonal groups occurred in the neonatal and pediatric units over the period studied, a fact highlighting the importance of improving hygiene practices and control measures of nosocomial infections in these units.

KeywordsNosocomial infections NICU PICU MRSA AbbreviationsMRSAMethicillin-resistant Staphylococcus aureus

NICUNeonatal intensive care units

PICUPediatric intensive care units

PBP 2aPenicillin-binding protein

SCCmecStaphylococcal cassette chromosome mec

PFGEPulsed-field gel electrophoresis

HC-FMBUniversity hospital of the Botucatu medical school

Electronic supplementary materialThe online version of this article doi:10.1186-s12941-014-0050-4 contains supplementary material, which is available to authorized users.

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Autor: Valéria Cataneli Pereira - Danilo Flªvio Moraes Riboli - Maria de Lourdes Ribeiro de Souza da Cunha

Fuente: https://link.springer.com/







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