Proteomic analysis of human placental syncytiotrophoblast microvesicles in preeclampsiaReportar como inadecuado




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Clinical Proteomics

, 11:40

First Online: 19 November 2014Received: 22 January 2014Accepted: 29 October 2014

Abstract

BackgroundPlacental syncytiotrophoblast microvesicles STBM are shed into the maternal circulation during normal pregnancy. STBM circulate in significantly increased amounts in preeclampsia PE and are considered to be among contributors to the exaggerated proinflammatory, procoagulant state of PE. However, protein composition of STBM in normal pregnancy and PE remains unknown. We therefore sought to determine the protein components of STBM and whether STBM protein expressions differ in preeclamptic and normal pregnancies.

Patients with PE n = 3 and normal pregnant controls n = 6 were recruited. STBM were prepared from placental explant culture supernatant. STBM proteins were analyzed by a combination of 1D Gel-LC-MS-MS. Protein expressions levels were quantified using spectral counts and validated by immunohistochemistry.

ResultsOver 400 proteins were identified in the STBM samples. Among these, 25 proteins were found to be differentially expressed in preeclampsia compared to healthy pregnant controls, including integrins, annexins and histones.

ConclusionSTBM proteins include those that are implicated in immune response, coagulation, oxidative stress, apoptosis as well as lipid metabolism pathways. Differential protein expressions of STBM suggest their pathophysiological relevance in PE.

KeywordsPlacental microvesicles Pregnancy Preeclampsia Proteome AbbreviationsSTBMSyncytiotrophoblast microvesicle

PEPreeclampsia

PLAPPlacental alkaline phosphatase

NTANanoparticle tracking analysis.

Electronic supplementary materialThe online version of this article doi:10.1186-1559-0275-11-40 contains supplementary material, which is available to authorized users.

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Autor: Sonia Baig - Narasimhan Kothandaraman - Jayapal Manikandan - Li Rong - Kim Huey EE - Jeffrey Hill - Chin Wee Lai - Wan Y

Fuente: https://link.springer.com/







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