Expression of enterotoxin-coding genes in methicillin-resistant Staphylococcus aureusstrains isolated from Mexican haemodialysis patientsReportar como inadecuado




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Annals of Clinical Microbiology and Antimicrobials

, 13:55

First Online: 25 November 2014Received: 21 July 2014Accepted: 05 November 2014

Abstract

BackgroundMethicillin-resistant Staphylococcus aureus MRSA causes severe catheter-related infections in haemodialysis patients ranging from local-site infections and septic thrombophlebitis to bacteraemia but the associated virulence factors and exotoxins remain unclear.

FindingsWe employed an in vitro infection model using reconstituted human epithelium RHE to analyse the expression profiles of 4 virulence genes and 12 exotoxin-coding virulence genes in 21 MRSA strains isolated from catheter-related infections in 21 Mexican patients undergoing haemodialysis.

All 21 strains 100% expressed the seg, seh, sei, eta, etb, or hla genes coding staphylococcal toxins. Eleven MRSA strains 52.3% expressed the sea gene coding staphylococcal enterotoxin A, and two strains 9.5% expressed the v8 gene coding serine protease. The tst, chp, and arcA genes coding toxic shock syndrome toxin 1, chemotaxis inhibitory protein, and arginine deiminase, respectively, were expressed in separate single strains 4.7%. The most frequent expression profile 42.8% of the strains comprised seg, seh, sei, eta, etb, and hla.

ConclusionIt is likely that the SEG, SEH, SEI, ETA, ETB, and Hla toxins may play a role in MRSA catheter-related infections. Consideration of these toxins in the development of a vaccine or as targets for monoclonal antibody therapy could provide an improved therapeutic strategy for the treatment of catheter-related infections in haemodialysis patients.

KeywordsMRSA, Haemodialysis catheter Enterotoxins  Download fulltext PDF



Autor: Gloria Luz Paniagua-Contreras - Eric Monroy-Pérez - Felipe Vaca-Paniagua - José Raymundo Rodríguez-Moctezuma - Sergio Va

Fuente: https://link.springer.com/







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