Anthraquinonyl glycoside facilitates the standardization of graphene electrodes for the impedance detection of lectinsReportar como inadecuado




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Chemistry Central Journal

, 8:67

Analytical Chemistry

Abstract

BackgroundConstruction of electrochemical impedance sensors by the self-assembly technique has become a promising strategy for the `label-free- detection of protein-ligand interactions. However, previous impedance sensors are devoid of an inherent electrochemical signal, which limits the standardization of the sensors for protein recognition in a reproducible manner.

ResultsWe designed and synthesized an anthraquinonyl glycoside AG where the anthraquinone AQ moiety can bind to the surface of a graphene-based working electrode while the glycoside serving as a ligand for lectin. By measuring the inherent voltammetric signal of AQ, the glycosides decorated on the working electrode could be simply quantified to obtain electrodes with a unified signal window. Subsequently, impedance analysis showed that the `standardized- electrodes gave a reproducible electrochemical response to a selective lectin with no signal variation in the presence of unselective proteins.

ConclusionAnthraquinone-modified ligands could be used to facilitate the standardization of electrochemical impedance sensors for the reproducible, selective analysis of ligand-protein interactions.

KeywordsAnthraquinone Graphene Glycoside Lectin Electrochemistry EIS Standardization Electronic supplementary materialThe online version of this article doi:10.1186-s13065-014-0067-y contains supplementary material, which is available to authorized users.

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Autor: Bi-Wen Zhu - Liang Cai - Xiao-Peng He - Guo-Rong Chen - Yi-Tao Long

Fuente: https://link.springer.com/



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